Product datasheet
Anti-Nkx2.5 antibody ab106923
1 Abreviews 2 Images
Overview
Product name
Anti-Nkx2.5 antibody
Description
Goat polyclonal to Nkx2.5
Specificity
ab106923 is expected to recognize all three reported isoforms (NP_004378.1;
NP_001159647.1; NP_001159648.1).
Tested applications
WB
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog
Immunogen
Synthetic peptide:
C-PRAYSDPDPAKDPR
, corresponding to internal sequence amino acids 94-107 of Human Nkx2.5 (NP_004378.1;
NP_001159647.1; NP_001159648.1).
Run BLAST with
Positive control
Run BLAST with
This antibody gave a positive signal in the following whole cell lysates: Human fetal heart; Mouse
heart; Rat Heart
General notes
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Purity
Immunogen affinity purified
Purification notes
ab106923 was purified from goat serum by ammonium sulphate precipitation followed by
antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab106923 in the following tested applications.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 45 kDa
(predicted molecular weight: 35 kDa).
Abcam recommends using 3% milk as the blocking agent.
Target
Function
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a
transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally
controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is
required for spleen development.
Tissue specificity
Expressed only in the heart.
Involvement in disease
Atrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital
Sequence similarities
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain.
Cellular localization
Nucleus.
Anti-Nkx2.5 antibody images
2
All lanes : Anti-Nkx2.5 antibody (ab106923)
at 1 µg/ml (Blocked with 3% Milk)
Lane 1 : Human Nkx2.5 recombinant protein
(tagged) at 0.1 µg
Lane 2 : HEK293 (Human) Whole Cell
Lysate (ab52256) at 20 µg
Lane 3 : PANC-1 (Human Pancreatic
Carcinoma) Whole Cell Lysate (negative
control) at 20 µg
Lane 4 : Heart (Mouse) Mouse Tissue Lysate
Western blot - Anti-Nkx2.5 antibody (ab106923)
at 20 µg
Lane 5 : Heart (Rat) Tissue Lysate at 20 µg
Lane 6 : Human Fetal Heart - Total Protein
Tissue Lysate at 20 µg
Secondary
Rabbit Anti-Goat IgG H&L (HRP) (ab97100)
at 1/20000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 35 kDa
Observed band size : 45 kDa
Exposure time : 4 minutes
This blot was produced using a 4-12% Bistris gel under the MOPS buffer system. The
gel was run at 200V for 50 minutes before
being transferred onto a Nitrocellulose
membrane at 30V for 70 minutes. The
membrane was then blocked for an hour
using 3% Milk before being incubated with
ab106923 overnight at 4°C. Antibody binding
was detected using an anti-rabbit antibody
conjugated to HRP, and visualised using ECL
development solution.
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Anti-Nkx2.5 antibody (ab106923) at 0.3 µg/ml
+ Human heart lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 35 kDa
Primary incubation was 1 hour.
Western blot - Nkx2.5 antibody (ab106923)
The observed molecular weight corresponds
to earlier findings in literature with different
antibodies (Shiojima et al, Circ Res. 1996
Nov;79(5):920-9.; PMID: 8888684).
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