Product datasheet
Anti-PON2 antibody ab40969
1 Abreviews 1 References 4 Images
Overview
Product name
Anti-PON2 antibody
Description
Rabbit polyclonal to PON2
Tested applications
IHC-P, WB, ICC/IF
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow, Dog
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human
PON2.Read Abcam's proprietary immunogen policy(Peptide available as ab41542.)
Positive control
This antibody gave a positive signal in the following Human whole cell lysates: Caco 2; HeLa Hydroxyurea Treated (48hr, 1µM). HeLa cell line in IF.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab40969 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
1/2000.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa
(predicted molecular weight: 40 kDa).
ICC/IF
Use a concentration of 5 µg/ml.
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Target
Function
Capable of hydrolyzing lactones and a number of aromatic carboxylic acid esters. Has
antioxidant activity. Is not associated with high density lipoprotein. Prevents LDL lipid
peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to
induce monocyte chemotaxis.
Tissue specificity
Widely expressed with highest expression in liver, lung, placenta, testis and heart.
Sequence similarities
Belongs to the paraoxonase family.
Post-translational
modifications
The signal sequence is not cleaved.
Cellular localization
Membrane.
Anti-PON2 antibody images
Immunohistochemistical detection of PON2
antibody (ab40969) on formaldehyde fixed
paraffin-embedded mosue kidney and lung
sectionsAntigen retrieval step: Heat
mediated. Buffer: Citric acid pH6.
Permeabilization: None. Blocking step: 1%
BSA for 10 mins @ 21°C. Primary antibody
incubated at 1/2000 for 2 hours @ 21°C in
TBS/BSA/azide. Secondary antibody: anti
Rabbit IgG Conjugated to Biotin (1/200).
Patterns of immunostaining are produced that
Immunohistochemistry (Formalin/PFA-fixed
appear to be very similar to those seen on the
paraffin-embedded sections) - PON2 antibody
HPA website, for these tissues. In the
(ab40969)
submitted composite image, both tissues
Carl Hobbs, King`s College London, United
Kingdom
show intense labelling. In the upper image of
kidney cortico-medullary junction, apical
membrane positivity is observed in
proximal/distal tubules. In the same tubules
globular intra-cytoplasmic positivity is also
observed. In the lower image of lung, positivity
is evident in the simple columnar epithelium of
the bronchioles and also in what appear to be
alveolar macrophages. This antibody should
be diluted further, under the conditions used.
2
All lanes : Anti-PON2 antibody (ab40969) at
1 µg/ml
Lane 1 : Caco 2 (Human colonic carcinoma
cell line) Whole Cell Lysate
Lane 2 : Hela Whole Cell Lysate Hydroxyurea Treated (48hr, 1uM)
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - Anti-PON2 antibody (ab40969)
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 40 kDa
Observed band size : 40 kDa
Additional bands at : 45 kDa (possible
glycosylated form),55 kDa. We are unsure as
to the identity of these extra bands.
Exposure time : 150 seconds
Anti-PON2 antibody (ab40969) at 1 µg/ml +
Recombinant Human PON2 protein
(ab53379) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Western blot - Anti-PON2 antibody (ab40969)
Exposure time : 30 seconds
3
ICC/IF image of ab40969 stained HeLa cells.
The cells were 100% methanol fixed (5 min)
and then incubated in 1%BSA / 10% normal
goat serum / 0.3M glycine for 1h to block nonspecific protein-protein interactions. The cells
were then incubated with the antibody
(ab40969, 5µg/ml) overnight at +4°C. The
secondary antibody (green) was ab96899,
DyLight® 488 goat anti-rabbit IgG (H+L) used
at a 1/250 dilution for 1h. Alexa Fluor® 594
Immunocytochemistry/ Immunofluorescence -
WGA was used to label plasma membranes
Anti-PON2 antibody (ab40969)
(red) at a 1/200 dilution for 1h. DAPI was
used to stain the cell nuclei (blue) at a
concentration of 1.43µM.
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