Product datasheet
Anti-Ferritin Heavy Chain antibody [EPR3005Y]
ab75972
8 Abreviews 1 References 8 Images
Overview
Product name
Anti-Ferritin Heavy Chain antibody [EPR3005Y]
Description
Rabbit monoclonal [EPR3005Y] to Ferritin Heavy Chain
Specificity
This antibody recognizes only the Ferritin heavy chain.
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
corresponding to Human Ferritin Heavy Chain aa 150-250 (C terminal).
Positive control
WB: Human fetal liver, SH-SY5Y, human brain and HeLa cell lysates. IHC: Human kidney, breast
and tonsil tissue.
General notes
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class
antibodies. As a result of this work we are pleased to now offer this antibody in purified
format. We are in the process of updating our datasheets. The purified format is
designated ‘PUR’ on our product labels. If you have any questions regarding this
update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with
these species. Please contact us for more information.
Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Avoid freeze / thaw cycle.
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity
Protein A purified
Clonality
Monoclonal
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Clone number
EPR3005Y
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab75972 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/500 - 1/2000. Detects a band of approximately 21 kDa (predicted molecular
weight: 21 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker
method before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF.
Target
Anti-Ferritin Heavy Chain antibody [EPR3005Y] images
Anti-Ferritin Heavy Chain antibody
[EPR3005Y] (ab75972) at 1/5000 dilution +
Human fetal liver at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the nonreduced form of IgG at 1/1000 dilution
Predicted band size : 21 kDa
Western blot - Anti-Ferritin Heavy Chain antibody
[EPR3005Y] (ab75972)
2
Anti-Ferritin Heavy Chain antibody
[EPR3005Y] (ab75972) at 1/2000 dilution +
HeLa (human cervix adenocarcinoma) whole
cell lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase
conjugated (ab97051) at 1/20000 dilution
Predicted band size : 21 kDa
Western blot - Anti-Ferritin Heavy Chain antibody
[EPR3005Y] (ab75972)
ab75972 staining Ferritin Heavy Chain in
human liver tissue sections by
Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embedded
sections). Tissue was fixed with
paraformaldehyde and antigen retrieval was
by heat mediation in a EDTA buffer. Samples
were incubated with primary antibody at a
dilution of 1/200. A goat anti-rabbit IgG H&L
(HRP) ab97051 was used as the secondary
antibody at a dilution of 1/500.
Immunohistochemistry - Anti-Ferritin Heavy Chain
antibody [EPR3005Y] (ab75972)
Negative control 1: PBS in place of primary
antibody.
3
Lanes 1 - 3 : Anti-Ferritin Heavy Chain
antibody [EPR3005Y] (ab75972) at 1/500
dilution (unpurified)
Lane 4 : Anti-Ferritin Heavy Chain antibody
[EPR3005Y] (ab75972) at 1/2000 dilution
(unpurified)
Lane 5 : Anti-Ferritin Heavy Chain antibody
[EPR3005Y] (ab75972) at 1/5000 dilution
(unpurified)
Western blot - Anti-Ferritin Heavy Chain antibody
[EPR3005Y] (ab75972)
Lane 1 : Human liver lysate
Lane 2 : SH-SY5Y lysate
Lane 3 : Human brain lysate
Lane 4 : Human brain lysate
Lane 5 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000
dilution
Predicted band size : 21 kDa
Observed band size : 21 kDa
Unpurified ab75972 at 1/100 dilution staining
Ferritin Heavy Chain in human kidney tissue,
using a HRP/AP polymerized secondary
antibody.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Ferritin Heavy
Chain antibody [EPR3005Y] (ab75972)
4
Unpurified ab75972 showing positive staining
in normal liver tissue.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Ferritin Heavy
Chain antibody [EPR3005Y] (ab75972)
Unpurified ab75972 showing positive staining
in normal breast tissue.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Ferritin Heavy
Chain antibody [EPR3005Y] (ab75972)
Unpurified ab75972 showing positive staining
in normal tonsil tissue.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Ferritin Heavy
Chain antibody [EPR3005Y] (ab75972)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
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