Product datasheet
Anti-FRMD7 antibody ab129246
1 Abreviews 2 Images
Overview
Product name
Anti-FRMD7 antibody
Description
Rabbit polyclonal to FRMD7
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Human
Immunogen
KLH conjugated synthetic peptide between amino acids 114-144 from the N-terminal region of
Human FRMD7 (NP_919253.1).
Positive control
HepG2 cell line lysate. Human stomach tissue.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% PBS
Purity
Immunogen affinity purified
Purification notes
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab129246 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/100 - 1/500. Predicted molecular weight: 82 kDa.
IHC-P
1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6
before commencing with IHC staining protocol.
Target
1
Function
Plays a role in neurite development (By similarity). May play a specific role in the control of eye
movement and gaze stability.
Tissue specificity
Expressed in liver, kidney, pancreas and at low levels in brain and heart. Expressed in
embryonic brain and developing neural retina.
Involvement in disease
Defects in FRMD7 are the cause of nystagmus congenital X-linked type 1 (NYS1) [MIM:310700].
NYS1 is a condition defined as conjugated, spontaneous and involuntary ocular oscillations that
appear at birth or during the first three months of life. Other associated features may include
mildly decreased visual acuity, strabismus, astigmatism, and occasionally head nodding.
Sequence similarities
Contains 1 FERM domain.
Developmental stage
In 37 day post-ovulation (dpo) embryos, expression is found in the mid- and hindbrain, regions
known to be involved in motor control of eye movement, and in the ventricular zone of the
forebrain. In 56 dpo embryos, expressed in the ventricular layer of the forebrain, midbrain,
cerebellar primordium, spinal cord and the developing neural retina. In later development, highly
expressed in postmitotic cells within the developing subplate and cortical plate.
Cellular localization
Cell projection. Cell projection > growth cone. In undifferentiated neurons, located in the actinrich regions of the cell body. In differentiated neurons, located in the actin-rich regions of the cell
body and primary neurite processes but is almost absent from secondary extensions arising
from the primary neurite. Also found at the actin-rich distal end of growth cones.
Anti-FRMD7 antibody images
Anti-FRMD7 antibody (ab129246) at 1/100
dilution + HepG2 cell line lysate at 35 µg
Predicted band size : 82 kDa
Western blot - Anti-FRMD7 antibody (ab129246)
ab129246 at 1/10 dilution, staining FRMD7 in
Formalin- fixed Paraffin-embedded Human
stomach tissue by Immunohistochemistry
followed by peroxidase conjugation of the
secondary antibody and DAB staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-FRMD7
antibody (ab129246)
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