Product datasheet
Anti-FGF9 antibody ab9743
2 Abreviews 2 References 2 Images
Overview
Product name
Anti-FGF9 antibody
Description
Rabbit polyclonal to FGF9
Tested applications
IHC-P, WB, ELISA, Neutralising
Species reactivity
Reacts with: Mouse, Rat
Immunogen
Highly pure (>98%) recombinant mFGF-9 (mouse Fibroblast Growth Factor-9).
Properties
Form
Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product
in liquid form it has already been reconstituted as described and no further reconstitution is
necessary.
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Storage buffer
PBS, pH 7.4, no preservative, sterile filtered
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Applications
Our Abpromise guarantee covers the use of ab9743 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
Use at an assay dependent dilution. PubMed: 17192470
WB
Use a concentration of 0.1 - 0.2 µg/ml.
ELISA
Use a concentration of 0.5 µg/ml. Allows the detection of 0.2 - 0.4 ng/well of
recombinant mFGF-9 (using 100µl/well antibody solution).
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Application
Abreviews
Neutralising
Notes
Use a concentration of 0.025 - 0.06 µg/ml. for one-half maximal inhibition [ND50]
of the biological activity of mFGF-9 (1.5 ng/ml).
Target
Function
May have a role in glial cell growth and differentiation during development, gliosis during repair
and regeneration of brain tissue after damage, differentiation and survival of neuronal cells, and
growth stimulation of glial tumors.
Tissue specificity
Glial cells.
Involvement in disease
Defects in FGF9 are the cause of multiple synostoses syndrome type 3 (SYNS3) [MIM:612961].
Multiple synostoses syndrome is an autosomal dominant condition characterized by progressive
joint fusions of the fingers, wrists, ankles and cervical spine, characteristic facies and
progressive conductive deafness.
Sequence similarities
Belongs to the heparin-binding growth factors family.
Post-translational
modifications
Three molecular species were found (30 kDa, 29 kDa and 25 kDa), cleaved at Leu-4, Val-13
and Ser-34 respectively. The smaller ones might be products of proteolytic digestion.
Furthermore, there may be a functional signal sequence in the 30 kDa species which is
uncleavable in the secretion step.
N-glycosylated.
Cellular localization
Secreted.
Anti-FGF9 antibody images
All lanes : Anti-FGF9 antibody (ab9743) at
1/500 dilution
Lane 1 : Recombinant FGF9
Lane 2 : Rat cerebral cortex membrane
whole tissue lysate. at 15 µg
Secondary
Mouse anti-rabbit IgG monoclonal at 1/1000
dilution
developed using the ECL technique
Western blot - FGF9 antibody (ab9743)
Exposure time : 30 seconds
This image is courtesy of an anonymous
Abreview
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ab9743 staining FGF9 in mouse lymph node
tissue by Immunohistochemistry
(Formalin/PFA-fixed paraffin-embedded
tissue sections). The sections were fixed in
paraformaldehye prior to blocking with 10%
serum for 1 hour at 20°C. The primary
antibody was diluted 1/100 and incubated
with the sample for 12 hours at 4°C. An HRPconjugated Goat anti-Rabbit polyclonal was
Immunohistochemistry (Formalin/PFA-fixed
used as the secondary antibody, diluted
paraffin-embedded sections) - FGF9 antibody
1/200.
(ab9743)
This image is courtesy of an Anonymous Abreview.
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