Product datasheet Anti-Dact3 antibody [2A5] ab81615 1 Image Overview Product name Anti-Dact3 antibody [2A5] Description Mouse monoclonal [2A5] to Dact3 Tested applications WB, ELISA Species reactivity Reacts with: Mouse, Human Immunogen Recombinant fragment, corresponding to amino acids 89-202 of Human Dact3 (NP_659493) Positive control Mouse brain and 293T cell lysates. Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle. Storage buffer Preservative: 0.1% Sodium Azide Constituents: PBS, pH 7.4 Purity Protein G purified Clonality Monoclonal Clone number 2A5 Isotype IgG1 Light chain type kappa Applications Our Abpromise guarantee covers the use of ab81615 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application WB Abreviews Notes 1/250 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). ELISA Use at an assay dependent dilution. Target 1 Sequence similarities Belongs to the dapper family. Domain The C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH (Dishevelled) family proteins. Anti-Dact3 antibody [2A5] images All lanes : Anti-Dact3 antibody [2A5] (ab81615) at 1/250 dilution Lane 1 : mouse brain cell lysate Lane 2 : 293T cell lysate Lysates/proteins at 50 µg per lane. Secondary goat anti-mouse HRP Western blot - Dact3 antibody [2A5] (ab81615) developed using the ECL technique Predicted band size : 65 kDa Observed band size : 65 kDa Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2