Anti-Dact3 antibody [2A5] ab81615 Product datasheet 1 Image Overview

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Product datasheet
Anti-Dact3 antibody [2A5] ab81615
1 Image
Overview
Product name
Anti-Dact3 antibody [2A5]
Description
Mouse monoclonal [2A5] to Dact3
Tested applications
WB, ELISA
Species reactivity
Reacts with: Mouse, Human
Immunogen
Recombinant fragment, corresponding to amino acids 89-202 of Human Dact3 (NP_659493)
Positive control
Mouse brain and 293T cell lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Purity
Protein G purified
Clonality
Monoclonal
Clone number
2A5
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab81615 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/250 - 1/500. Detects a band of approximately 65 kDa (predicted molecular
weight: 65 kDa).
ELISA
Use at an assay dependent dilution.
Target
1
Sequence similarities
Belongs to the dapper family.
Domain
The C-terminal PDZ-binding motif may mediate interaction with the PDZ domains of DSH
(Dishevelled) family proteins.
Anti-Dact3 antibody [2A5] images
All lanes : Anti-Dact3 antibody [2A5]
(ab81615) at 1/250 dilution
Lane 1 : mouse brain cell lysate
Lane 2 : 293T cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
goat anti-mouse HRP
Western blot - Dact3 antibody [2A5] (ab81615)
developed using the ECL technique
Predicted band size : 65 kDa
Observed band size : 65 kDa
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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