Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
Anti-Aldehyde dehydrogenase 10 antibody
Mouse polyclonal to Aldehyde dehydrogenase 10
WB, ICC/IF
Reacts with: Human full-length human Aldehyde dehydrogenase 10. (NP_001026976.1).
human liver lysate, Aldehyde dehydrogenase 10 transfected 293T cell line, HeLa cells.
Properties
Form
Storage instructions
Storage buffer
Purity
Clonality
Isotype
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: None
Constituents: PBS, pH 7.2
Protein A purified
Polyclonal
IgG
Applications
Our Abpromise guarantee covers the use of ab72774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB
ICC/IF
Application notes ICC/IF: Use at a concentration of 10 µg/ml. Note: ab72774 needs to be Protein A purified before use.
WB: 1/500 - 1/1000. Predicted molecular weight: 55 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Target
Function
Involvement in disease
Sequence similarities
Cellular localization
Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length.
Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS) [MIM:270200]. SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization).
Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects.
Belongs to the aldehyde dehydrogenase family.
Endoplasmic reticulum membrane.
Anti-Aldehyde dehydrogenase 10 antibody images
Anti-Aldehyde dehydrogenase 10 antibody
(ab72774) at 1/500 dilution + human liver lysate at 50 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Western blot - Aldehyde dehydrogenase 10 antibody (ab72774)
Predicted band size : 55 kDa
Observed band size : 50 kDa
Western blot - Aldehyde dehydrogenase 10 antibody (ab72774)
All lanes : Anti-Aldehyde dehydrogenase 10 antibody (ab72774) at 1/500 dilution
Lane 1 : Aldehyde dehydrogenase 10 transfected 293T cell line
Lane 2 : non transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 55 kDa
Observed band size : 51 kDa
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Immunofluorescence analysis of Aldehyde dehydrogenase 10 expression in HeLa cells using 10 µg/ml purified ab72774
Immunocytochemistry/ Immunofluorescence -
Aldehyde dehydrogenase 10 antibody (ab72774)
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