Overview
Product name
Description
Tested applications
Species reactivity
Immunogen
Positive control
General notes
Anti-Aldehyde dehydrogenase 10 antibody [2A7]
Mouse monoclonal [2A7] to Aldehyde dehydrogenase 10
WB, IHC-P, Flow Cyt
Reacts with: Human
Recombinant full length Human Aldehyde dehydrogenase 10 produced in HEK293T cells
(NP_001026976).
HEK293T cells transfected with pCMV6-ENTRY Aldehyde dehydrogenase 10, Human breast adenocarcinoma, endometrium and endometrium adenocarcinoma tissues.
Dilute in PBS (pH7.3) before use. Stable for 12 months from date of receipt.
Properties
Form
Storage instructions
Storage buffer
Purity
Purification notes
Clonality
Clone number
Isotype
Liquid
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Protein A purified
Purified from mouse ascites fluid by affinity chromatography.
Monoclonal
2A7
IgG2b
Applications
Our Abpromise guarantee covers the use of ab123910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes
WB 1/500. Predicted molecular weight: 55 kDa.
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Application
IHC-P
Flow Cyt
Abreviews Notes
1/150.
1/100. ab170192 -Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
Target
Function
Involvement in disease
Sequence similarities
Cellular localization
Catalyzes the oxidation of long-chain aliphatic aldehydes to fatty acids. Active on a variety of saturated and unsaturated aliphatic aldehydes between 6 and 24 carbons in length.
Defects in ALDH3A2 are the cause of Sjoegren-Larsson syndrome (SLS) [MIM:270200]. SLS is an autosomal recessive neurocutaneous disorder characterized by a combination of severe mental retardation, spastic di- or tetraplegia and congenital ichthyosis (increased keratinization).
Ichthyosis is usually evident at birth, neurologic symptoms appear in the first or second year of life. Most patients have an IQ of less than 60. Additional clinical features include glistening white spots on the retina, seizures, short stature and speech defects.
Belongs to the aldehyde dehydrogenase family.
Endoplasmic reticulum membrane.
Anti-Aldehyde dehydrogenase 10 antibody [2A7] images
All lanes : Anti-Aldehyde dehydrogenase 10 antibody [2A7] (ab123910) at 1/500 dilution
Lane 1 : HEK293T cells transfected with pCMV6-ENTRY control cDNA
Lane 2 : HEK293T cells transfected pCMV6pCMV6-ENTRY Aldehyde dehydrogenase 10 cDNA
Lysates/proteins at 5 µg per lane.
Western blot - Anti-Aldehyde dehydrogenase 10 antibody [2A7] (ab123910)
Predicted band size : 55 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone
(RC204857)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldehyde dehydrogenase 10 antibody [2A7] (ab123910) ab123910 at 1/150 dilution staining Aldehyde dehydrogenase 10 in Paraffin-embedded
Human breast adenocarcinoma tissue by
Immunohistochemistry.
ab123910 at 1/150 dilution staining Aldehyde dehydrogenase 10 in Paraffin-embedded
Human endometrium tissue by
Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldehyde dehydrogenase 10 antibody [2A7] (ab123910) ab123910 at 1/150 dilution staining Aldehyde dehydrogenase 10 in Paraffin-embedded
Human endometrium adenocarcinoma tissue by Immunohistochemistry.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aldehyde dehydrogenase 10 antibody [2A7] (ab123910)
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ab123910 at 1/100 dilution staining Aldehyde dehydrogenase 10 in HEK293T cells transfected with either pCMV6-ENTRY
Aldehyde dehydrogenase 10 (Red) or empty vector control plasmid (Blue) by flow cytometry.
Flow Cytometry - Anti-Aldehyde dehydrogenase
10 antibody [2A7] (ab123910)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
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