Product datasheet
Anti-SCYL1 antibody ab76851
1 Abreviews 4 Images
Overview
Product name
Anti-SCYL1 antibody
Description
Mouse monoclonal to SCYL1
Tested applications
ICC/IF, Flow Cyt, IHC-Fr, WB, ELISA
Species reactivity
Reacts with: Mouse, Rat, Human, Recombinant Fragment
Immunogen
Recombinant fragment: DHKSSKSPES DWSSWEAEGS WEQGWQEPSS QEPPPDGTRL
ASEYNWGGPE SSDKGDPFAT LSARPSTQDR SRLSWPGRSA RSGGGRWRPN
APRGRWPRAP , corresponding to amino acids 373-472 of Human SCYL1 (AAH09967).
Run BLAST with
Positive control
Run BLAST with
HeLa nuclear lysates
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab76851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
Use a concentration of 1 µg/ml.
Flow Cyt
Use 0.5µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use
as an isotype control with this antibody.
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Application
Abreviews
Notes
IHC-Fr
1/50.
WB
1/500 - 1/1000. Detects a band of approximately 75 kDa (predicted molecular
weight: 90 kDa).
ELISA
Use at an assay dependent concentration.
Target
Function
Regulates COPI-mediated retrograde traffic. Has no detectable kinase activity in vitro.
Isoform 6 acts as transcriptional activator. It binds to three different types of GC-rich DNA
binding sites (box-A, -B and -C) in the beta-polymerase promoter region. It also binds to the
TERT promoter region.
Tissue specificity
Ubiquitous.
Sequence similarities
Belongs to the protein kinase superfamily.
Contains 3 HEAT repeats.
Contains 1 protein kinase domain.
Domain
The protein kinase domain is predicted to be catalytically inactive.
Cellular localization
Nucleus; Cytoplasm > cytoskeleton > centrosome. Endoplasmic reticulum-Golgi intermediate
compartment. Golgi apparatus > cis-Golgi network. Localized to the Endoplasmic reticulumGolgi intermediate and cis-Golgi in an ARF1-independent manner; Cytoplasm. Cytoplasm >
cytoskeleton > centrosome. Cytoplasmic during interphase and centrosomal during mitosis, it
localizes to the centrosomes in a microtubule-independent manner and Cytoplasm. Cytoplasmic
throughout the cell cycle.
Anti-SCYL1 antibody images
Anti-SCYL1 antibody (ab76851) at 1/500
dilution + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
secondary antibody at 1/5000 dilution
Predicted band size : 90 kDa
Western blot - SCYL1 antibody (ab76851)
Observed band size : 40 kDa
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Anti-SCYL1 antibody (ab76851) at 1/500
dilution + HeLa nuclear extract at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
secondary antibody at 1/2500 dilution
Predicted band size : 90 kDa
Western blot - SCYL1 antibody (ab76851)
Observed band size : 75 kDa
Additional bands at : 70 kDa. We are
unsure as to the identity of these extra bands.
ICC/IF image of ab76851 stained HeLa cells.
The cells were 4% formaldehyde fixed (10
min) and then incubated in 1%BSA / 10%
normal goat serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody (ab76851, 1µg/ml) overnight
at +4°C. The secondary antibody (green) was
Immunocytochemistry/ Immunofluorescence -
Alexa Fluor® 488 goat anti-mouse IgG (H+L)
SCYL1 antibody (ab76851)
used at a 1/1000 dilution for 1h. Alexa Fluor®
594 WGA was used to label plasma
membranes (red) at a 1/200 dilution for 1h.
DAPI was used to stain the cell nuclei (blue)
at a concentration of 1.43µM.
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Overlay histogram showing HeLa cells
stained with ab76851 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
Flow Cytometry-Anti-SCYL1 antibody(ab76851)
(ab76851, 0.5µg/1x106 cells) for 30 min at
22ºC. The secondary antibody used was
DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG2a [ICIGG2A] (ab91361,
1µg/1x106 cells) used under the same
conditions. Unlabelled sample (blue line).
Acquisition of >5,000 events were collected
using a 20mW Argon ion laser (488nm) and
525/30 bandpass filter.
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