Product datasheet
Anti-CRMP4 antibody [EPR7593(B)] ab126787
2 Abreviews 3 Images
Overview
Product name
Anti-CRMP4 antibody [EPR7593(B)]
Description
Rabbit monoclonal [EPR7593(B)] to CRMP4
Tested applications
ICC/IF, WB, IP
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
A synthetic peptide, corresponding to residues in Human CRMP4 (UniProt Q14195).
Positive control
Human hippocampus and fetal brain lysates.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 7.50 x 10 -10 M
Learn more about KD
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR7593(B)
Isotype
IgG
Applications
1
Our Abpromise guarantee covers the use of ab126787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
1/100.
WB
1/1000 - 1/10000. Detects a band of approximately 62 kDa (predicted molecular
weight: 62 kDa).
IP
Application notes
1/10 - 1/100.
Is unsuitable for Flow Cyt or IHC-P.
Target
Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton.
Plays a role in axon guidance, neuronal growth cone collapse and cell migration.
Tissue specificity
Mainly expressed in heart and skeletal muscle. Also strongly expressed in fetal brain and spinal
cord.
Sequence similarities
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
Post-translational
modifications
Phosphorylation on Ser-522 by DYRK2 promotes subsequent phosphorylation on Thr-509, Thr514 and Ser-518 by GSK3.
Cellular localization
Cytoplasm. Cell projection > growth cone. Colocalizes with synaptic vesicle protein 2 in the
central region of the growth cone.
Anti-CRMP4 antibody [EPR7593(B)] images
ab126787 staining CRMP4
in Human medulloblasma cell line by ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with methanol/acetone
mixture, permeabilized with 0.25% Triton X100 in PBS and blocked with 5% serum for
30 minutes at 22°C. Samples were incubated
with primary antibody (1/100 in PBS + 5%
Goat serum) for 16 hours at 4°C. An Alexa
Immunocytochemistry/ Immunofluorescence -
Fluor® 555-conjugated Goat anti-rabbit IgG
Anti-CRMP4 antibody [EPR7593(B)] (ab126787)
polyclonal (1/1000) was used as the
This image is courtesy of an Abreview submitted by
Radhi Praba Velayutham
secondary antibody.
2
All lanes : Anti-CRMP4 antibody
[EPR7593(B)] (ab126787) at 1/1000 dilution
Lane 1 : Human hippocampus lysate
Lane 2 : Human fetal brain lysate
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - Anti-CRMP4 antibody
HRP labelled goat anti-rabbit at 1/2000
[EPR7593(B)] (ab126787)
dilution
Predicted band size : 62 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-CRMP4 antibody [EPR7593(B)]
(ab126787)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3