Product datasheet
Anti-FIG4 antibody ab97621
1 Image
Overview
Product name
Anti-FIG4 antibody
Description
Rabbit polyclonal to FIG4
Tested applications
WB
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Monkey
Immunogen
Recombinant protein fragment corresponding to a region within amino acids 135 and 400 of
FIG4 (NP_055660)
Positive control
HCT116 and HeLa cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab97621 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/500 - 1/3000. Predicted molecular weight: 104 kDa.
Target
Function
The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of
phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). In vitro, hydrolyzes all three D51
phosphorylated polyphosphoinositide substrates in the order PtdIns(4,5)P2 > PtdIns(3,5)P2 >
PtdIns(3,4,5)P3. Plays a role in the biogenesis of endosome carrier vesicles (ECV) /
multivesicular bodies (MVB) transport intermediates from early endosomes.
Involvement in disease
Defects in FIG4 are the cause of Charcot-Marie-Tooth disease type 4J (CMT4J) [MIM:611228].
CMT4J is a recessive demyelinating, severe form of Charcot-Marie-Tooth disease, the most
common inherited disorder of the peripheral nervous system. Charcot-Marie-Tooth disease is
classified in two main groups on the basis of electrophysiologic properties and histopathology:
primary peripheral demyelinating neuropathies characterized by severely reduced motor nerve
conduction velocities (NCVs) (less than 38m/s) and segmental demyelination and remyelination,
and primary peripheral axonal neuropathies characterized by normal or mildly reduced NCVs
and chronic axonal degeneration and regeneration on nerve biopsy.
Defects in FIG4 are the cause of amyotrophic lateral sclerosis type 11 (ALS11) [MIM:612577].
ALS is a neurodegenerative disorder affecting upper motor neurons in the brain and lower motor
neurons in the brain stem and spinal cord, resulting in fatal paralysis. Sensory abnormalities are
absent. Death usually occurs within 2 to 5 years. The etiology of amyotrophic lateral sclerosis is
likely to be multifactorial, involving both genetic and environmental factors. The disease is
inherited in 5-10%.
Sequence similarities
Contains 1 SAC domain.
Cellular localization
Endosome membrane. Localization requires VAC14 and PIKFYVE.
Anti-FIG4 antibody images
Anti-FIG4 antibody (ab97621) at 1/1000
dilution + HCT116 whole cell lysate at 30 µg
Predicted band size : 104 kDa
Western blot - FIG4 antibody (ab97621)
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