Product datasheet
Anti-PHOX2A antibody ab168392
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Overview
Product name
Anti-PHOX2A antibody
Description
Mouse polyclonal to PHOX2A
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Gorilla,
Orangutan
Immunogen
Recombinant full length protein, corresponding to amino acids 1-284 of Human PHOX2A
(NP_005160.2).
Run BLAST with
Run BLAST with
Positive control
PHOX2A transfected 293T cell line lysate, HeLa cells
General notes
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term.
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab168392 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 30 kDa.
ICC/IF
Use a concentration of 10 µg/ml. For IHC and IF applications, antibody
purification with Protein A will be needed prior to use.
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Target
Function
May be involved in regulating the specificity of expression of the catecholamine biosynthetic
genes. Acts as a transcription activator/factor. Could maintain the noradrenergic phenotype.
Involvement in disease
Defects in PHOX2A are the cause of congenital fibrosis of extraocular muscles type 2
(CFEOM2) [MIM:602078]. CFEOM encompasses several different inherited strabismus
syndromes characterized by congenital restrictive ophthalmoplegia affecting extraocular
muscles innervated by the oculomotor and/or trochlear nerves. CFEOM is characterized clinically
by anchoring of the eyes in downward gaze, ptosis, and backward tilt of the head. CFEOM2 may
result from the aberrant development of the oculomotor (nIII), trochlear (nIV) and abducens (nVI)
cranial nerve nuclei.
Sequence similarities
Belongs to the paired homeobox family.
Contains 1 homeobox DNA-binding domain.
Cellular localization
Nucleus.
Anti-PHOX2A antibody images
All lanes : Anti-PHOX2A antibody
(ab168392) at 1 µg/ml
Lane 1 : PHOX2A transfected 293T cell line
lysate
Lane 2 : Non-transfected 293T cell line lysate
Western blot - Anti-PHOX2A antibody (ab168392)
Predicted band size : 30 kDa
Immunofluorescent analysis of HeLa cells
labeling PHOX2A with ab168392 at 10 µg/ml.
Immunocytochemistry/ Immunofluorescence Anti-PHOX2A antibody (ab168392)
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