Product datasheet
Anti-Laminin antibody [LAM-89] ab49726
1 Abreviews 2 References 2 Images
Overview
Product name
Anti-Laminin antibody [LAM-89]
Description
Mouse monoclonal [LAM-89] to Laminin
Specificity
ab49726 recognises Laminin. It does not cross react with collagen IV, fibronectin, vitronectin or
chondroitin sulphate types A, B and C.
Tested applications
IHC-Fr, IHC-P, Sandwich ELISA, WB
Species reactivity
Reacts with: Cat, Human, Pig
Does not react with: Mouse, Rat, Sheep, Rabbit, Goat, Chicken, Guinea pig, Dog, Xenopus
laevis, Carp, Lizard, Snake
Immunogen
Purified human laminin.
Positive control
Kidney, skeletal muscle or small intestine, human placenta.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites diluted in PBS with 1% BSA
Purity
Ascites
Clonality
Monoclonal
Clone number
LAM-89
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab49726 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-Fr
1/50 - 1/100.
IHC-P
Use at an assay dependent concentration.
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Application
Abreviews
Sandwich ELISA
Notes
Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Rabbit
polyclonal to Laminin (ab23753). For sandwich ELISA, use this antibody as
Detection at 0.5µg/ml with Ab23753 as Capture.
WB
Use at an assay dependent concentration. Predicted molecular weight: 337 kDa.
Target
Function
Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment,
migration and organization of cells into tissues during embryonic development by interacting with
other extracellular matrix components.
Tissue specificity
Broadly expressed in: skin, heart, lung, and the reproductive tracts.
Sequence similarities
Contains 11 laminin EGF-like domains.
Contains 1 laminin IV type A domain.
Contains 1 laminin N-terminal domain.
Domain
The alpha-helical domains I and II are thought to interact with other laminin chains to form a
coiled coil structure.
Domain IV is globular.
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane.
Anti-Laminin antibody [LAM-89] images
Paraffin section of human placenta:
immunohistochemical staining for laminin
using 1/50 ab49726. Note staining of
basement membranes of blood
vessels.Paraffin section of human placenta:
immunohistochemical staining for laminin
using 1/50 ab49726. Note staining of
basement membranes of blood vessels.
Immunohistochemistry (Paraffin-embedded
sections) - Laminin antibody [LAM-89] (ab49726)
Standard curve for Laminin; dilution range
1pg/ml to 1µg/ml using Capture Antibody
Rabbit polyclonal to Laminin (ab23753) at
5µg/ml and Detector Antibody Mouse
monoclonal [LAM-89] to Laminin (ab49726)
at 0.5µg/ml.
Sandwich ELISA - Laminin antibody [LAM-89]
(ab49726)
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