Product datasheet
Anti-KIF5B antibody [KN-03] ab11883
1 Abreviews 1 Image
Overview
Product name
Anti-KIF5B antibody [KN-03]
Description
Mouse monoclonal [KN-03] to KIF5B
Specificity
The antibody reacts with heavy chain of conventional kinesin associated with vesicles and with
lower affinity with denaturated molecule. The antibody recognizes kinesin bound to taxolstabilized microtubules.
Tested applications
ICC/IF, ICC
Species reactivity
Reacts with: Mouse, Rat, Human, Pig
Immunogen
Enriched fraction of porcine brain kinesin.
Epitope
Epitope mapping has revealed that antibodies ab11882 and ab11883 react with different sets
of fragments.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Purity
Purified IgM
Purification notes
Purified from ascites by thiophilic adsorption-affinity chromatography.
Clonality
Monoclonal
Clone number
KN-03
Isotype
IgM
Applications
Our Abpromise guarantee covers the use of ab11883 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
ICC/IF
ICC
Application notes
ICC: Use at an assay dependent dilution.
1
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Microtubule-dependent motor required for normal distribution of mitochondria and lysosomes.
Sequence similarities
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin
subfamily.
Contains 1 kinesin motor domain.
Domain
Composed of three structural domains: a large globular N-terminal domain which is responsible
for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical
coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal
domain which interacts with other proteins (such as the kinesin light chains), vesicles and
membranous organelles.
Cellular localization
Cytoplasm, cytoskeleton. Uniformly distributed between soma and neurites in hippocampal
neurons.
Anti-KIF5B antibody [KN-03] images
ab11883 staining rat neuronal stem cells by
ICC/IF. Cells were PFA fixed and
permeabilized in 0.5% Saponin, prior to
blocking with 10% serum for 1 hour at 21°C.
The primary antibody was diluted 1/500 with
PBS + 1% FCS and incubated with the
sample for 18 hours at 4°C. A Cy5®
conjugated goat anti-mouse IgG antibody was
used as the secondary.
Immunocytochemistry/ Immunofluorescence Anti-KIF5B antibody [KN-03] (ab11883)
This image is courtesy of an Abreview submitted by
Dr Dominik Schelshorn
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
2
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3