Product datasheet
Anti-APH1a antibody ab12104
1 Abreviews 2 References 2 Images
Overview
Product name
Anti-APH1a antibody
Description
Rabbit polyclonal to APH1a
Specificity
Recognises both isoforms of APH1a (encoded on chromosome 1)
Tested applications
IHC-FoFr, WB
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide derived from residues 50 - 150 of Human APH1a. Read Abcam's proprietary
immunogen policy.
Positive control
This antibody gave a positive signal in human and mouse brain lysates in WB and in mouse
brain tissue sections in IF.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab12104 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-FoFr
Use at an assay dependent concentration.
WB
Use a concentration of 2 µg/ml. Detects a band of approximately 30 kDa
(predicted molecular weight: 30 kDa).
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Target
Function
Essential subunit of the gamma-secretase complex, an endoprotease complex that catalyzes the
intramembrane cleavage of integral proteins such as Notch receptors and APP (beta-amyloid
precursor protein). It probably represents a stabilizing cofactor for the presenilin homodimer that
promotes the formation of a stable complex.
Tissue specificity
Widely expressed. Expressed in leukocytes, lung, placenta, small intestine, liver, kidney, spleen
thymus, skeletal muscle, heart and brain. Isoform 1 and isoform 2 are nearly expressed at the
same level.
Sequence similarities
Belongs to the APH-1 family.
Cellular localization
Endoplasmic reticulum membrane. Golgi apparatus > Golgi stack membrane. Predominantly
located in the endoplasmic reticulum and in the cis-Golgi.
Anti-APH1a antibody images
All lanes : Anti-APH1a antibody (ab12104)
at 1/500 dilution
Lane 1 : Brain (Human) tissue lysate
Lane 2 : Brain (Mouse) tissue lysate
Lane 3 : Brain (Human) tissue lysate with
Human APH1a peptide (ab12394) at 1 µg/ml
Lane 4 : Brain (Mouse) tissue lysate with
Human APH1a peptide (ab12394) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Western blot - Anti-APH1a antibody (ab12104)
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab7090) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 30 kDa
Observed band size : 30 kDa
Additional bands at : 21 kDa (possible
isoform),75 kDa. We are unsure as to the
identity of these extra bands.
Exposure time : 30 seconds
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ab12104 staining APH1a in mouse brain
tissue section (cortex) by
Immunohistochemistry (PFA perfusion fixed
frozen sections). Tissue from 4% PFA
perfused animals underwent overnight fixation
in 4% paraformaldehyde, cryoprotected in
30% sucrose and cut using cryostat.The
primary antibody was diluted, 1/1000 (PBS +
0.3% Triton X100) and incubated with sample
for 18 hours at 20°C. An abcam antibody
ab60314, Chromeo488 conjugated goat
Immunohistochemistry (PFA perfusion fixed
polyclonal to rabbit IgG, diluted 1/1000 was
frozen sections) - APH1a antibody (ab12104)
used as secondary. ab12104 produces a very
This image is courtesy of an Abreview submitted by
Dr Sophie Pezet
pretty staining in neuronal processes in the
cortex and the hippocampus.
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