Product datasheet
Anti-Synaptotagmin 1 antibody ab77314
1 Abreviews 1 References 4 Images
Overview
Product name
Anti-Synaptotagmin 1 antibody
Description
Mouse monoclonal to Synaptotagmin 1
Tested applications
Flow Cyt, WB, ELISA
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Recombinant full length human Synaptotagmin 1 protein (AAH58917) with a 26kDa tag
Positive control
Jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2a
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab77314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Flow Cyt
Abreviews
Notes
Use 0.5-1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for
use as an isotype control with this antibody.
WB
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 48 kDa.
ELISA
Use at an assay dependent concentration. Detection limit is approximately 3
ng/ml as a capture antibody when used against the immunogen.
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Target
Function
May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at
the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the
presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction
between synaptotagmin and putative receptors for activated protein kinase C has also been
reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these
are neurexins, syntaxin and AP2.
Sequence similarities
Belongs to the synaptotagmin family.
Contains 2 C2 domains.
Domain
The first C2 domain mediates Ca(2+)-dependent phospholipid binding.
The second C2 domain mediates interaction with SV2A and STN2.
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle >
secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin
granules.
Anti-Synaptotagmin 1 antibody images
Anti-Synaptotagmin 1 antibody (ab77314) at
5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
at 1/5000 dilution
developed using the ECL technique
Western blot - Synaptotagmin 1 antibody
(ab77314)
Predicted band size : 48 kDa
Observed band size : 65 kDa
The observed band size may not correspond
to the predicted protein molecular weight as
the immunogen (recombinant tagged protein)
was used as the sample. Molecular weight of
the tag alone is 26 kDa.
Anti-Synaptotagmin 1 antibody (ab77314) at
5 µg/ml + Jurkat cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate
at 1/2500 dilution
developed using the ECL technique
Western blot - Synaptotagmin 1 antibody
(ab77314)
Predicted band size : 48 kDa
Observed band size : 38 kDa
2
ab77314 was used as a capture antibody at
3ng/ml followed by incubation with the
immunogen (recombinant protein) at the
indicated concentration.
ELISA - Synaptotagmin 1 antibody (ab77314)
Overlay histogram showing SH-SY5Y cells
stained with ab77314 (red line). The cells
were fixed with 80% methanol (5 min) and
then permeabilized with 0.1% PBS-Tween for
20 min. The cells were then incubated in 1x
PBS / 10% normal goat serum / 0.3M glycine
to block non-specific protein-protein
interactions followed by the antibody
Flow Cytometry-Anti-Synaptotagmin 1
antibody(ab77314)
(ab77314, 1µg/1x106 cells) for 30 min at
22ºC. The secondary antibody used was
DyLight® 488 goat anti-mouse IgG (H+L)
(ab96879) at 1/500 dilution for 30 min at
22ºC. Isotype control antibody (black line) was
mouse IgG2a [ICIGG2A] (ab91361,
2µg/1x106 cells) used under the same
conditions. Acquisition of >5,000 events was
performed.
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