Product datasheet
Anti-GST3 / GST pi antibody ab53943
2 Abreviews 1 References 3 Images
Overview
Product name
Anti-GST3 / GST pi antibody
Description
Goat polyclonal to GST3 / GST pi
Specificity
ab53943 is specific for GST pi.
Tested applications
WB, IHC-P, IP, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Rabbit, Hamster, Human
Immunogen
Full length native protein (purified) GST3/ GST pi (Rat)
Positive control
This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: HCT116.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.7% BSA
Purity
IgG fraction
Purification notes
IgG fraction was prepared from the antisera using the caprylic acid precipitation method.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab53943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/3000. Detects a band of approximately 31 kDa (predicted molecular weight: 23
kDa).
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before
commencing with IHC staining protocol.
IP
Use at an assay dependent concentration. PubMed: 23741294
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Application
Abreviews
ICC/IF
Notes
1/200.
Target
Function
Conjugation of reduced glutathione to a wide number of exogenous and endogenous
hydrophobic electrophiles.
Sequence similarities
Belongs to the GST superfamily. Pi family.
Contains 1 GST C-terminal domain.
Contains 1 GST N-terminal domain.
Anti-GST3 / GST pi antibody images
IHC image of ab53943 staining in human
normal liver formalin fixed paraffin embedded
tissue section, performed on a Leica BondTM
system using the standard protocol B. The
section was pre-treated using heat mediated
antigen retrieval with sodium citrate buffer
(pH6, epitope retrieval solution 1) for 20 mins.
The section was then incubated with
ab53943, 5µg/ml, for 15 mins at room
Immunohistochemistry (Formalin/PFA-fixed
temperature and detected using an HRP
paraffin-embedded sections) - Anti-GST3 / GST pi
conjugated compact polymer system. DAB
antibody (ab53943)
was used as the chromogen. The section was
then counterstained with haematoxylin and
mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
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Predicted band size : 23 kDa
Western blot - Anti-GST3 / GST pi antibody
(ab53943)
ab53943 stained HCT116 cells. The cells
were 4% formaldehyde fixed (10 min) and
then incubated in 1%BSA / 10% normal
donkey serum / 0.3M glycine in 0.1% PBSTween for 1h to permeabilise the cells and
block non-specific protein-protein
interactions. The cells were then incubated
with the antibody ab53943 at 1/200 dilution
overnight at +4°C. The secondary antibody
Immunocytochemistry/ Immunofluorescence -
(green) was DyLight® 488 donkey anti- goat
Anti-GST3 / GST pi antibody (ab53943)
IgG (H+L) used at a 1/1000 dilution for 1h.
Alexa Fluor® 594 WGA was used to label
plasma membranes (red) at a 1/200 dilution
for 1h. DAPI was used to stain the cell nuclei
(blue) at a concentration of 1.43µM.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
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Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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