Product datasheet
Anti-GAD65 antibody [144] ab85866
3 Abreviews 2 References 2 Images
Overview
Product name
Anti-GAD65 antibody [144]
Description
Mouse monoclonal [144] to GAD65
Tested applications
WB, IP, Flow Cyt, RIA
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide:
GSGFWSFGSEDGSGDSEN
conjugated to KLH, corresponding to amino acids 4-22 of Human GAD65
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Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS
Purity
Protein G purified
Clonality
Monoclonal
Clone number
144
Myeloma
Sp2/0-Ag14
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab85866 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa.
IP
Use at an assay dependent concentration.
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Application
Abreviews
Flow Cyt
Notes
Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is
suitable for use as an isotype control with this antibody.
RIA
Use at an assay dependent concentration.
Target
Function
Catalyzes the production of GABA.
Sequence similarities
Belongs to the group II decarboxylase family.
Post-translational
modifications
Phosphorylated; which does not affect kinetic parameters or subcellular location.
Palmitoylated; which is required for presynaptic clustering.
Cellular localization
Cytoplasm > cytosol. Cytoplasmic vesicle. Cell junction > synapse > presynaptic cell membrane.
Golgi apparatus membrane. Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of
Golgi membranes and presynaptic clusters.
Anti-GAD65 antibody [144] images
Predicted band size : 65 kDa
Observed band size : ~65-70 kDa
This image is courtesy of an anonymous
Abreview
Western blot - GAD65 antibody [144] (ab85866)
ab85866 staining GAD65 in murine brain
synaptosomes by Flow cytometry.
Cells were homogenized and centrifuged then
fixed in paraformaldehyde and permeabilized
using PBS Tween. Size gating was used.
Primary antibody used at 0.5µg/ml and
incubated for 30 minutes at 24°C. The
secondary antibody used was an undiluted,
Alexa Fluor®488 conjugated goat anti-rabbit
Flow Cytometry - GAD65 antibody [144]
IgG.
(ab85866)
Image courtesy of an anonymous Abreview.
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