Product datasheet
Anti-CCKAR antibody ab77269
1 References 2 Images
Overview
Product name
Anti-CCKAR antibody
Description
Goat polyclonal to CCKAR
Tested applications
WB, IHC-P, ELISA, ICC/IF
Species reactivity
Reacts with: Rat, Human
Immunogen
Synthetic peptide:
C-FEASQKKSAKERKPST
, corresponding to internal sequence amino acids 242-257 of Human CCKAR (NP_000721.1).
Run BLAST with
Positive control
Run BLAST with
Human Pancreas tissue. This antibody gave a positive result when used in the following
formaldehyde fixed cell lines: CaCo2
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Purity
Immunogen affinity purified
Purification notes
ab77269 was purified from goat serum by ammonium sulphate precipitation followed by antigen
affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab77269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
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Application
Abreviews
WB
Notes
Use at an assay dependent concentration. Preliminary experiments in Human
Pancreas lysates gave no specific signal but low background (at antibody
concentration up to 1µg/ml).
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before
commencing with IHC staining protocol.
ELISA
Use at an assay dependent concentration. Antibody detection limit for peptide
ELISA 1:4000
ICC/IF
Use a concentration of 5 µg/ml.
Target
Relevance
The cholecystokinin (CCK) family of peptide hormones have been implicated in numerous
important physiologic events. These appear to be mediated through 2 general classes of
receptors, A (CCKAR)and B (CCKBR), based on their binding affinities for CCK/gastrin family
peptides. Through binding to class A receptors, CCK is a major physiologic mediator of
gallbladder contraction and pancreatic enzyme secretion. It appears to play a role in slowing
gastric emptying, relaxation of the sphincter of Oddi, and potentiation of insulin secretion.
Further, it has been implicated as a mediator of pancreatic growth and tumorigenesis. Class A
receptors have also been described in the anterior pituitary, myenteric plexus, and regions of the
central nervous system, where they have been implicated in the pathogenesis of feeding
disorders, Parkinson disease, schizophrenia, and drug addiction.
Cellular localization
Integral membrane protein
Anti-CCKAR antibody images
ICC/IF image of ab77269 stained CaCo2
cells. The cells were 4% formaldehyde fixed
(10 min) and then incubated in 1%BSA / 10%
normal donkey serum / 0.3M glycine in 0.1%
PBS-Tween for 1h to permeabilise the cells
and block non-specific protein-protein
interactions. The cells were then incubated
with the antibody ab77269 at 5µg/ml
overnight at +4°C. The secondary antibody
(green) was DyLight® 488 donkey anti- goat
(ab96931) IgG (H+L) used at a 1/250 dilution
Immunocytochemistry/ Immunofluorescence -
for 1h. Alexa Fluor® 594 WGA was used to
Anti-CCKAR antibody (ab77269)
label plasma membranes (red) at a 1/200
dilution for 1h. DAPI was used to stain the cell
nuclei (blue) at a concentration of 1.43µM.
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ab77269 at 10µg/ml staining CCKAR in
human pancreas by Immunohistochemistry,
Paraffin-embedded tissue (note cytoplasmic
staining of some cells in the islets of
Langerhans).
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - CCKAR antibody
(ab77269)
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