Product datasheet
Anti-MRF antibody - Oligodendrocyte Marker ab85464
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Overview
Product name
Anti-MRF antibody - Oligodendrocyte Marker
Description
Rabbit polyclonal to MRF - Oligodendrocyte Marker
This product is a fast track antibody. It has been affinity purified and shows high
titre values against the immunizing peptide by ELISA.
Read the terms of use »
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat, Rabbit
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1100 to the C-terminus of
Human MRF.Read Abcam's proprietary immunogen policy
Positive control
This antibody gave a positive signal in the following Mouse tissue lysates: Brain day 0;
Embryonic brain E14; Embryonic brain E16.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Fast track antibodies constitute a diverse group of products that have been released to accelerate your research, but are not yet fully
characterized. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA).
Fast track terms of use
Application
WB
Abreviews
Notes
Use a concentration of 1 µg/ml. Detects a band of approximately 123 kDa
(predicted molecular weight: 123 kDa).
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Target
Function
Transcription regulator required for expression of central nervous system (CNS) myelin genes
such as MBP and MOG, thereby playing a central role in oligodendrocyte maturation and CNS
myelination. Probably acts as a transcription factor that directly binds DNA and activates
expression of CNS myelin genes.
Tissue specificity
Expressed in lung, ARPE-19 cell line, brainstem, uterus and, to a lesser extent, in basal ganglion
and liver. Weakly expressed in cerebellum and retina.
Sequence similarities
Belongs to the MRF family.
Contains 1 NDT80 DNA-binding domain.
Domain
A transmembrane region is predicted by sequence analysis tools. However, the protein is
nuclear and probably acts as a transcription factor. It is therefore unclear whether the predicted
transmembrane region is a hydrophobic region that does not insert into membranes or whether it
is a real transmembrane region that may be cleaved when acting as transcription regulator.
Cellular localization
Membrane. Nucleus.
Anti-MRF antibody - Oligodendrocyte Marker images
This Fast-Track antibody is not yet fully characterised. These images represent inconclusive preliminary data.
All lanes : Anti-MRF antibody Oligodendrocyte Marker (ab85464) at 1/250
dilution
Lane 1 : Brain (Mouse) Tissue Lysate - 0
days old (ab7188)
Lane 2 : E14 Mouse Embryo Brain Tissue
Lysate
Lane 3 : E16 Ms Embryo Brain Tissue
Lysate
Western blot - Anti-MRF antibody -
Lysates/proteins at 10 µg per lane.
Oligodendrocyte Marker (ab85464)
Secondary
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 123 kDa
Observed band size : 123 kDa
Additional bands at : 22 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 4 minutes
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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