Product datasheet
Anti-TUBA4A (acetyl K40) antibody [6-11B-1] ab11323
5 Abreviews 5 References 4 Images
Overview
Product name
Anti-TUBA4A (acetyl K40) antibody [6-11B-1]
Description
Mouse monoclonal [6-11B-1] to TUBA4A (acetyl K40)
Specificity
ab11323 recognizes an epitope located on the a3 isoform of Chlamydomonas axonemal atubulin, within four residues of Lys-40 when this amino acid is acetylated. A sequence very
similar to the one detected by ab11323 in Chlamydomonas is found in the majority of a-tubulins,
but the corresponding region is markedly divergent in some a-tubulin isoforms from chicken,
Drosophila and yeast. The antibody has been used to detect acetylated a-tubulins in several
organisms. Occasionally, the epitope recognized by the antibody may be absent or masked as it
is in the kangaroo rat epithelial-like cell line PtK2.
Tested applications
WB, ELISA, Dot Blot, RIA, IHC-P, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, Pig, Xenopus laevis,
Caenorhabditis elegans, Chlamydomonas reinhardtii, Sea Urchin
Immunogen
Acetylated TUBA4A from the outer arm of sea urchin sperm axonemes.
General notes
Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged
storage, clarify the solution by centrifugation before use.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites fluid
Purity
Ascites
Clonality
Monoclonal
Clone number
6-11B-1
Isotype
IgG2b
Applications
Our Abpromise guarantee covers the use of ab11323 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
1/2000. This dilution was determined using bovine or rat brain preparation.
ELISA
Use at an assay dependent concentration.
Dot Blot
Use at an assay dependent concentration.
RIA
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. PubMed: 23269434
ICC/IF
Use at an assay dependent concentration.
Target
Function
Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an
exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
Sequence similarities
Belongs to the tubulin family.
Post-translational
modifications
Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs
exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl
group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in
human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and
flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the
mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and
lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of
such modifications is still unclear but they regulate the assembly and dynamics of axonemal
microtubules.
Acetylation of alpha-tubulins at Lys-40 stabilizes microtubules and affects affinity and
processivity of microtubule motors. This modification has a role in multiple cellular functions,
ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking
and signaling.
Cellular localization
Cytoplasm > cytoskeleton.
Anti-TUBA4A (acetyl K40) antibody [6-11B-1] images
ab11323 at 1/800 staining dog kidney cells
by ICC/IF. The cells were paraformaldehyde
fixed and blocked with BSA prior to
incubation with the antibody for 1 hour. An
Alexa-Fluor ® 488 conjugated goat antimouse IgG was used as the secondary.
Immunocytochemistry/ Immunofluorescence Anti-TUBA4A (acetyl K40) antibody [6-11B-1]
(ab11323)
This image is courtesy of an anonymous Abreview
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All lanes : Anti-TUBA4A (acetyl K40)
antibody [6-11B-1] (ab11323) at 1/1000
dilution
Lane 1 : Whole animal lysate prepared from
Western blot - Anti-TUBA4A (acetyl K40) antibody
[6-11B-1] (ab11323)
wild type (N2) C. Elegans worms.
Lane 2 : Whole animal lysate prepared from
elpc-1 C. Elegans worms.
Lane 3 : Whole animal lysate prepared from
elpc-3 C. Elegans worms.
Lane 4 : Whole animal lysate prepared from
mec-12 (e1605) C. Elegans worms.
Lane 5 : Whole animal lysate prepared from
mec-12 (e1607) C. Elegans worms.
Lysates/proteins at 20 µg per lane.
Image from Chen C et al, PLoS Genet. 2009
Jul;5(7):e1000561. Epub 2009 Jul 10, fig S9.
Anti-TUBA4A (acetyl K40) antibody [6-11B-1]
(ab11323) at 1/2000 dilution (in PBST + 2.5%
milk for 16 hours at 4°C) + Whole cell lysate of
human IHP1 cells at 30 µg
Secondary
An HRP-conjugated Goat anti-mouse IgG
monoclonal at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Western blot - Anti-TUBA4A (acetyl K40) antibody
[6-11B-1] (ab11323)
Observed band size : 55 kDa
Exposure time : 20 seconds
This image is courtesy of an anonymous
Abreview
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ab11323 staining Tubulin (acetyl K40) in
human glioblastoma tumor sphere cells by
ICC/IF
(Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde,
permeabilized with Triton X-100 0.1% in PBS
and blocked with 0.5% BSA for 20 minutes at
room temperature. Samples were incubated
with primary antibody (1/100 in PBS + 0.5%
BSA) for 1 hour at 4°C. An Alexa Fluor®488conjugated Goat anti-mouse IgG polyclonal
Immunocytochemistry/ Immunofluorescence -
(1/400) was used as the secondary antibody.
Anti-TUBA4A (acetyl K40) antibody [6-11B-1]
Nuclei were counterstained with DAPI (blue).
(ab11323)
This image is courtesy of an abreview submitted by
Lars Ruether
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