Product datasheet
Anti-CRMP1 antibody [EPR14520(B)] - N-terminal
ab180517
1 Abreviews 3 Images
Overview
Product name
Anti-CRMP1 antibody [EPR14520(B)] - N-terminal
Description
Rabbit monoclonal [EPR14520(B)] to CRMP1 - N-terminal
Tested applications
WB, IHC-P
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within
Human CRMP1 aa 50-150 (N terminal). The exact sequence is proprietary.
Database link: Q14194
Positive control
Human cerebellum lysate, Fetal brain lysate, SH-SY5Y cell lysate, Paraffin-embedded Human
brain sections.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR14520(B)
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab180517 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 62 kDa.
IHC-P
1/250 - 1/500.
Target
Function
Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton.
Plays a role in axon guidance, invasive growth and cell migration.
Tissue specificity
Brain.
Sequence similarities
Belongs to the DHOase family. Hydantoinase/dihydropyrimidinase subfamily.
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Cytoplasm > cytoskeleton > spindle.
Associated with centrosomes and the mitotic spindle during metaphase.
Anti-CRMP1 antibody [EPR14520(B)] - N-terminal images
All lanes : Anti-CRMP1 antibody
[EPR14520(B)] - N-terminal (ab180517) at
1/20000 dilution
Lane 1 : SH-SY5Y lysate
Lane 2 : Fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636)
Western blot - Anti-CRMP1 [EPR14520(B)]
at 1/500 dilution
antibody - N-terminal (ab180517)
Predicted band size : 62 kDa
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Anti-CRMP1 antibody [EPR14520(B)] - Nterminal (ab180517) at 1/5000 dilution +
Human cerebellum lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab136636)
at 1/500 dilution
Predicted band size : 62 kDa
Western blot - Anti-CRMP1 [EPR14520(B)]
antibody - N-terminal (ab180517)
Immunohistochemical analysis of paraffinembedded Human brain tissue labeling
CRMP1 with ab180517 at 1/500 dilution.
Anti-rabbit HRP seconday antibody was used
and counterstaining with Hematoxylin was
performed.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-CRMP1
[EPR14520(B)] antibody - N-terminal (ab180517)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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