Product datasheet
Anti-Pax2 antibody ab38738
3 Abreviews 2 References 4 Images
Overview
Product name
Anti-Pax2 antibody
Description
Rabbit polyclonal to Pax2
Tested applications
WB, IHC-Fr, ICC/IF
Species reactivity
Reacts with: Mouse, Zebrafish
Predicted to work with: Chicken, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Mouse
Pax2.Read Abcam's proprietary immunogen policy(Peptide available as ab39108.)
Positive control
This antibody gives a positive signal in the following lysates: Mouse Fetal lysate - 14 days old
tissue lysate Brain (Mouse) Whole Cell Lysate - normal tissue, 0 days old Mouse Embryonic
Fibroblast 1 Whole Cell Lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or 80°C. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab38738 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/250. Detects a band of approximately 44 kDa (predicted molecular weight: 44
kDa).
IHC-Fr
1/100.
ICC/IF
Use a concentration of 1 µg/ml.
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Target
Relevance
Pax2 is a transcription factor critically required during the development of the nervous and
excretory systems, including the midbrain, hindbrain, spinal cord, eye, ear and urogenital tract.
Like other products of the Pax gene family, Pax2 encodes a conserved 128 amino acid paired
box DNA-binding domain in the N-terminal portion of the molecule. Function: Probable
transcription factor that may have a role in kidney cell differentiation. Has a critical role in the
development of the urogenital tract, the eyes, and the CNS. Tissue specificity: Expressed in
primitive cells of the kidney, ureter, eye, ear and central nervous system.
Cellular localization
Nuclear
Anti-Pax2 antibody images
Anti-Pax2 antibody (ab38738)
IHC-Fr image of Pax2 staining on zebrafish
retina sections using ab38738 (1:100). The
sections were paraformaldehyde and
permeabilized using Triton-X. Antigen
retrieval was performed using Tris-HCl and
blocking was perfomed using 5% BSA for 1
hour at 23°C. ab38738 was diluted 1:100 and
incubated with the sections for 16 hours at
4°C. The secondary antibody was goat
polyclonal to rabbit IgG conjugated to Alexa
Fluor 488 (1:1000).
Immunohistochemistry (Frozen sections) - AntiPax2 antibody (ab38738)
Image courtesy Dr. Ryan MacDonald (Cambridge
University, United Kingdom)
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All lanes : Anti-Pax2 antibody (ab38738) at 1
µg/ml
Lane 1 : Brain (Mouse) Tissue Lysate - 0
days old (ab7188)
Lane 2 : Mouse Embryonic Fibroblast 1
Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - Anti-Pax2 antibody (ab38738)
Goat Anti-Rabbit IgG H&L (HRP)
preadsorbed at 1/50000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 17 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 8 minutes
This blot was produced using a 4-12% Bistris gel under the MOPS buffer system. The
gel was run at 200V for 50 minutes before
being transferred onto a Nitrocellulose
membrane at 30V for 70 minutes. The
membrane was then blocked for an hour
using 2% Bovine Serum Albumin before
being incubated with ab38738 overnight at
4°C. Antibody binding was detected using an
anti-rabbit antibody conjugated to HRP, and
visualised using ECL development solution
ab133406.
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All lanes : Anti-Pax2 antibody (ab38738) at
1/250 dilution
Lane 1 : Mouse Fetal lysate - 14 Days old
Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate - 0
days old (ab7188)
Lane 3 : Mouse Embryonic Fibroblast 1
Whole Cell Lysate
Lane 4 : Skeletal Muscle (Mouse) Tissue
Lysate (ab29711)
Western blot - Pax2 antibody (ab38738)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG
(H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 44 kDa
Observed band size : 44 kDa
Additional bands at : 25 kDa,70 kDa. We
are unsure as to the identity of these extra
bands.
Pax2 is not expressed in skeletal muscle.
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