Product datasheet
Anti-Ephrin A1 antibody [EPR1785(2)] ab124911
2 Images
Overview
Product name
Anti-Ephrin A1 antibody [EPR1785(2)]
Description
Rabbit monoclonal [EPR1785(2)] to Ephrin A1
Tested applications
WB
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat
Immunogen
Synthetic peptide from the N terminal region of Human Ephrin A1.
Positive control
HUVEC (treated with TNF alpha), HUVEC (untreated) and MCF7 cell lysates.
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 1.59 x 10 -10 M
Learn more about KD
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR1785(2)
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab124911 in the following tested applications.
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The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/1000 - 1/10000. Detects a band of approximately 24 kDa (predicted molecular
weight: 24 kDa).
Application notes
Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
Target
Function
Plays an important role in angiogenesis and tumor neovascularization. The recruitement of
VAV2, VAV3 and PI3-kinase p85 subunit by phosphorylated EPHA2 is critical for EFNA1induced RAC1 GTPase activation and vascular endothelial cell migration and assembly (By
similarity). Exerts anti-oncogenic effects in tumor cells through activation and down-regulation of
EPHA2. Activates EPHA2 by inducing tyrosine phosphorylation which leads to its internalization
and degradation. Acts as a negative regulator in the tumorigenesis of gliomas by downregulating EPHA2 and FAK. Can evoke collapse of embryonic neuronal growth cones.
Tissue specificity
Brain. Down-regulated in primary glioma tissues compared to the normal tissues. The soluble
monomeric form is expressed in the glioblastoma multiforme (GBM) and breast cancer cells (at
protein level).
Sequence similarities
Belongs to the ephrin family.
Post-translational
modifications
Undergo proteolysis by a metalloprotease to give rise to a soluble monomeric form.
Cellular localization
Secreted and Cell membrane.
Anti-Ephrin A1 antibody [EPR1785(2)] images
All lanes : Anti-Ephrin A1 antibody
[EPR1785(2)] (ab124911) at 1/1000 dilution
Lane 1 : HUVEC cell lysate, treated with TNF
alpha
Lane 2 : HUVEC cell lysate
Lane 3 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Western blot - Anti-Ephrin A1 antibody
Goat anti-Rabbit HRP at 1/2000 dilution
[EPR1785(2)] (ab124911)
Predicted band size : 24 kDa
Observed band size : 24 kDa
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Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-Ephrin A1 antibody [EPR1785(2)]
(ab124911)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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