Product datasheet
Anti-NeuN antibody - Neuronal Marker ab134014
4 Abreviews 1 References 4 Images
Overview
Product name
Anti-NeuN antibody - Neuronal Marker
Description
Chicken polyclonal to NeuN - Neuronal Marker
Tested applications
IHC-P, IHC-Fr, ICC, WB
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthetic peptide corresponding to NeuN (C terminal) conjugated to Keyhole Limpet
Haemocyanin (KLH). Corresponding to a region within Human (NP_001076044) and Mouse
(NP_001020102).
Positive control
This product gave a signal in Western Blot in the following tissue lysates: Mouse Brain; Rat Brain
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C.
Avoid freeze / thaw cycle.
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.9% Sodium chloride
Purification notes
ab134014 was affinity-purified from the IgY fraction prepared from eggs liad by immunised hens.
The final product is a mixture of affinity-purified NeuN antibodies (25 µg/ml), NeuN IgY fraction
(10 mg/ml).
Clonality
Polyclonal
Isotype
IgY
Applications
Our Abpromise guarantee covers the use of ab134014 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
1/1000 - 1/2000.
IHC-Fr
1/1000 - 1/2000.
ICC
1/1000 - 1/2000.
1
Application
Abreviews
WB
Notes
1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 34
kDa).
Target
Function
RNA-binding protein that regulates alternative splicing events.
Sequence similarities
Contains 1 RRM (RNA recognition motif) domain.
Cellular localization
Nucleus. Cytoplasm.
Anti-NeuN antibody - Neuronal Marker images
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) analysis of adult
mouse cerebral cortex tissue labelling NeuN
(red) with ab134014. Tissue was fixed with
4% paraformaldehyde. Green - rabbit antiBeta-Tubulin 3.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-NeuN antibody
- Neuronal Marker (ab134014)
All lanes : Anti-NeuN antibody - Neuronal
Marker (ab134014) at 1/1000 dilution
Lane 1 : Mouse Brain
Lane 2 : Rat Brain
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Western blot - Anti-NeuN antibody - Neuronal
Predicted band size : 34 kDa
Marker (ab134014)
Observed band size : 42 kDa
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Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) analysis of adult
mouse CA1 hippocampus tissue labelling
NeuN (red) with ab134014. Tissue was fixed
with 4% paraformaldehyde. Green - rabbit
anti-Beta-Tubulin 3.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-NeuN antibody
- Neuronal Marker (ab134014)
Immunohistochemistry (Frozen sections)
analysis of e18 mouse cochlear ganglion
tissue staining NeuN (green) with ab134014.
Tissue was fixed with 4% paraformaldehyde.
Red - rabbit anti-Beta-Tubulin 3.
Immunohistochemistry (Frozen sections) - AntiNeuN antibody - Neuronal Marker (ab134014)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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