Product datasheet
Anti-S100 antibody - Astrocyte Marker ab868
13 Abreviews 15 References 4 Images
Overview
Product name
Anti-S100 antibody - Astrocyte Marker
Description
Rabbit polyclonal to S100 - Astrocyte Marker
Specificity
This antibody recognizes all the S-100 isoforms, proteins of 21-24kDa identified as the A and B
subunits of S100 protein. It stains schwannomas, ependymomas, astrogliomas, and almost all
benign and malignant melanomas and their metastases. S100 protein is also expressed in the
antigen presenting cells such as the Langerhans cells in skin and interdigitating reticulum cells in
the paracortex of lymph nodes.
Tested applications
IHC (PFA fixed), IHC-FoFr, IHC-P, WB, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Cow, Human, Pig
Immunogen
Recombinant full length protein (Cow).
Positive control
Melanoma or Schwannoma
General notes
Contains a proprietary preservative that is not sodium azide or thimerosal
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze /
thaw cycle.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Da Vinci Green Diluent, pH 7.3
Purity
Whole antiserum
Clonality
Polyclonal
Myeloma
unknown
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab868 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC (PFA fixed)
Abreviews
Notes
Use at an assay dependent concentration. PubMed: 25184527
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Application
Abreviews
Notes
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20633557
IHC-P
1/100 - 1/200.
WB
Use at an assay dependent concentration. PubMed: 18032704
ICC/IF
Use at an assay dependent concentration. PubMed: 19593677
Target
Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist
for both ions on each monomer. Physiological concentrations of potassium ion antagonize the
binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
Tissue specificity
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains.
Cellular localization
Cytoplasm.
Anti-S100 antibody - Astrocyte Marker images
ab868 staining mouse brain tissue sections
by IHC-P. Sections were formaldehyde fixed
and permeabilized in 0.1% Triton-X100 in
blocking solution prior to blocking in 100%
BioGenex Power Block Universal Blocking
Reagent for 10 minutes at 22°C. The primary
antibody was diluted 1/1000 in 1% BSA in
PBS pH 7.4 and incubated with the section
for 1 hour at 22°C. A HRP polymer
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - S100 antibody Astrocyte Marker (ab868)
This image is courtesy of an anonymous Abreview
conjugated anti-rabbit antibody was used as
the secondary. A tyramide Alexa Fluor® 594
conjugate was added and the signal was
visualized using a fluorescent microscope
and a rhodamine filter
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ab868 staining S100 in mouse cerebellum by
immunohistochemistry (formalin/PFA-fixed
paraffin-embedded sections). Cells were
paraformaldehyde fixed prior to blocking in
5% serum for 30 minutes at 25°C. The
primary antibody was diluted 1/100 and
incubated with the sample for 16 hours at
4°C. Alexa fluor® 488 goat polyclonal to
rabbit Ig, diluted 1/400, was used as the
secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - S100 antibody Astrocyte Marker (ab868)
This image is courtesy of an Abreview submitted by P
Vig
ab868 staining S100 - Astrocyte Marker in
Mouse hippocampus tissue sections by
Immunohistochemistry (IHC-P paraformaldehyde-fixed, paraffin-embedded
sections). Tissue was fixed with
paraformaldehyde. Samples were incubated
with primary antibody (1/500) for 12 hours at
4°C. An undiluted HRP-conjugated polyclonal
was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - S100 antibody Astrocyte Marker (ab868)
This image is courtesy of an Abreview submitted by
Dr Markus Kipp
Immunohistochemical analysis of formalinfixed paraffin-embedded Melanoma section
labelling S100 with ab868 at 1/100 - 1/200
dilution. The tissue was counterstained
with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-S100 antibody
- Astrocyte Marker (ab868)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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