Product datasheet
Anti-pan Macrophage antibody [Ki-M2R] ab15637
1 References 1 Image
Overview
Product name
Anti-pan Macrophage antibody [Ki-M2R]
Description
Mouse monoclonal [Ki-M2R] to pan Macrophage
Specificity
Rat mature macrophages.
Tested applications
IHC-Fr, IHC-P
Species reactivity
Reacts with: Rat
Immunogen
Tissue/ cell preparation; Rat peritoneal macrophages
Epitope
This antibody recognises a surface antigen of 45kD molecular weight as a single band under
reducing conditions. The epitope is resistant to different fixations and paraffin processing.
Positive control
Rat Pancreas tissue slides. Liver (Kuppfer cells), spleen (macrophages of the red pulp), lymph
nodes (macrophages of the pulp and sinus, tingible body macrophages), lung (approx. 50% of
alveolar macrophages) and connective tissue (histiocytes) are also positively stained.
General notes
Recognizes typical tissue macrophages and specifically discriminates between monocytes (KiM2R-) and macrophages(Ki-M2R+). It stains all resident macrophages including those of bone
marrow, lymphatic sinuses, lymphoid follicles, splenic red pulp, and Kupffer cells in the liver.
Langerhans cells, interdigitating reticulum cells, and dendritic reticulum cells of lymphoid follicles
are not stained. Ki-M2R is a reliable tool for indicating macrophage differentiation in vivo and in
vitro.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 10mg/ml BSA. pH 7.2
Purity
Immunogen affinity purified
Primary antibody notes
Recognizes typical tissue macrophages and specifically discriminates between monocytes (KiM2R-) and macrophages(Ki-M2R+). It stains all resident macrophages including those of bone
marrow, lymphatic sinuses, lymphoid follicles, splenic red pulp, and Kupffer cells in the liver.
Langerhans cells, interdigitating reticulum cells, and dendritic reticulum cells of lymphoid follicles
are not stained. Ki-M2R is a reliable tool for indicating macrophage differentiation in vivo and in
vitro.
Clonality
Monoclonal
1
Clone number
Ki-M2R
Isotype
IgG1
Applications
Our Abpromise guarantee covers the use of ab15637 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-Fr
Use a concentration of 1 µg/ml.
IHC-P
Use a concentration of 2 µg/ml. Perform enzymatic antigen retrieval before
commencing with IHC staining protocol.
Target
Relevance
The antigen is found on a subpopullation of most tissue fixed macrophages. It is quite
predominant on red pulp macrophages and interdigitating cells in the outer periarteriolar
lymphatic sheath of the spleen, the inner border of marginal sinus of lymph nodes, and alveolar
macrophages. Langerhans cells of the skin and twisted spindle-shaped cells of the omentum are
detected by the antibody. It is absent from sinusoidal endothelial cells and parenchymal cells.
Cellular localization
Cell Membrane
Anti-pan Macrophage antibody [Ki-M2R] images
IHC-fr using Ab1537
Immunohistochemistry (Frozen sections) - pan
Macrophage antibody (ab15637)
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