Product datasheet
Anti-D6 antibody ab178503
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Overview
Product name
Anti-D6 antibody
Description
Rabbit polyclonal to D6
Tested applications
IHC-P, WB, ICC/IF
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide within Human D6 (C terminal). The exact sequence is proprietary.
Database link: O00590
Positive control
Human Lung respiratory epithelium; HepG2 cell extracts; COS7 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab178503 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
IHC-P
Abreviews
Notes
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with
citrate buffer pH 6 before commencing with IHC staining protocol.
WB
1/500 - 1/1000. Predicted molecular weight: 43 kDa.
ICC/IF
1/100 - 1/500.
Target
Relevance
Chemokines and their receptor-mediated signal transduction are critical for the recruitment of
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effector immune cells to the inflammation site. Chemokine receptor D6 is expressed in a range
of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells
and overexpression in vascular tumors suggested its function in chemokine-driven recirculation
of leukocytes and possible chemokine effects on the development and growth of vascular
tumors. This receptor appears to bind the majority of beta-chemokine family members.
Cellular localization
Cell Membrane
Anti-D6 antibody images
All lanes : Anti-D6 antibody (ab178503) at
1/500 dilution
Lane 1 : HepG2 cell extracts
Lane 2 : HepG2 cell extracts with synthesized
peptide
Western blot - Anti-D6 antibody - C-terminal
(ab178503)
Predicted band size : 43 kDa
Immunohistochemical analysis of formalinfixed, paraffin-embedded Human lung
respiratory epithelium tissue labeling D6 with
ab178503 at 5 µg/ml.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-D6 antibody C-terminal (ab178503)
Immunofluorescent analysis of COS7 cells
labeling D6 with ab178503 at 1/100
dilution. The image on the right is treated with
the synthesized peptide.
Immunocytochemistry/ Immunofluorescence Anti-D6 antibody - C-terminal (ab178503)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
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Valid for 12 months from date of delivery
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Terms and conditions
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