Product datasheet Anti-PI 3 Kinase p110 delta antibody [EPR386] ab109006 2 References 1 Image Overview Product name Anti-PI 3 Kinase p110 delta antibody [EPR386] Description Rabbit monoclonal [EPR386] to PI 3 Kinase p110 delta Tested applications WB, ICC Species reactivity Reacts with: Mouse, Rat, Human Immunogen A synthetic peptide corresponding to residues in Human PI3 Kinase p110 delta. Positive control Jurkat, Raji, K562, RAW 264.7, Rat brain, Rat kidney and Rat spleen lysates. General notes This product is a recombinant rabbit monoclonal antibody. Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487. Alternative versions available: Anti-PI 3 Kinase p110 delta antibody (HRP) [EPR386] (ab200773) Properties Form Liquid Storage instructions Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. Storage buffer pH: 7.40 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol, 0.05% BSA Purity Tissue culture supernatant Clonality Monoclonal Clone number EPR386 Isotype IgG Applications Our Abpromise guarantee covers the use of ab109006 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. 1 Application Abreviews Notes WB 1/1000 - 1/10000. Predicted molecular weight: 119 kDa. ICC 1/100 - 1/250. Application notes Is unsuitable for Flow Cyt,IHC-P or IP. Target Tissue specificity Expressed predominantly in leukocytes. Pathway Phospholipid metabolism; phosphatidylinositol phosphate biosynthesis. Sequence similarities Belongs to the PI3/PI4-kinase family. Contains 1 PI3K/PI4K domain. Post-translational modifications Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity. Anti-PI 3 Kinase p110 delta antibody [EPR386] images All lanes : Anti-PI 3 Kinase p110 delta antibody [EPR386] (ab109006) at 1/1000 dilution Lane 1 : Jurkat cell lysate Lane 2 : Raji cell lysate Lane 3 : K562 cell lysate Western blot - PI 3 Kinase p110 delta antibody [EPR386] (ab109006) Lane 4 : RAW 264.7 cell lysate Lane 5 : Rat brain tissue lysate Lane 6 : Rat kidney tissue lysate Lane 7 : Rat spleen tissue lysate Lysates/proteins at 10 µg per lane. Predicted band size : 119 kDa Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. 2 Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3