Product datasheet
Anti-67kDa Laminin Receptor antibody [EPR8468]
ab133775
4 Images
Overview
Product name
Anti-67kDa Laminin Receptor antibody [EPR8468]
Description
Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor
Tested applications
WB, IHC-P, Flow Cyt
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in Human 67kDa Laminin Receptor (UniProt ID:
P08865)
Positive control
WB: Lysates of K562, HeLa, HepG2, C6, RAW 264.7, PC 12, NIH/3T3 IHC-P: Human brain
tissue, Human kidney tissue
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 6.70 x 10 -11 M
Learn more about KD
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR8468
Isotype
IgG
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Applications
Our Abpromise guarantee covers the use of ab133775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular
weight: 33 kDa).
IHC-P
1/250 - 1/500.
Flow Cyt
1/100 - 1/500. ab172730-Rabbit monoclonal IgG, is suitable for use as an
isotype control with this antibody.
Application notes
Is unsuitable for ICC or IP.
Target
Function
Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the
processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of
40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell
adhesion to the basement membrane and in the consequent activation of signaling transduction
pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a
PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands,
including the pathogenic prion protein, viruses, and bacteria.
Sequence similarities
Belongs to the ribosomal protein S2P family.
Post-translational
modifications
Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin
receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may
provide a mechanism for membrane association.
Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a
mechanism to alter cell-extracellular matrix interactions.
Cellular localization
Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane,
with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at
the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B
in the cell membrane.
Anti-67kDa Laminin Receptor antibody [EPR8468] images
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All lanes : Anti-67kDa Laminin Receptor
antibody [EPR8468] (ab133775) at 1/1000
dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : C6 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lane 6 : PC12 cell lysate
Lane 7 : NIH/3T3 cell lysate
Western blot - Anti-67kDa Laminin Receptor
antibody [EPR8468] (ab133775)
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled Goat anti Rabbit IgG at 1/2000
dilution
Predicted band size : 33 kDa
Observed band size : 43 kDa
ab133775, at 1/250, staining 67kDa Laminin
Receptor in paraffin-embedded Human brain
tissue using immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-67kDa Laminin
Receptor antibody [EPR8468] (ab133775)
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ab133775, at 1/250, staining 67kDa Laminin
Receptor in paraffin-embedded Human
kidney tissue using immunohistochemical
analysis.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-67kDa Laminin
Receptor antibody [EPR8468] (ab133775)
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-67kDa Laminin Receptor antibody
[EPR8468](ab133775)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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