School of Life Sciences M EDIA LO CAT IO NS The Media Preparation Service is based at our Gibbet Hill Campus, Room M021. Near to School of Life Sciences Reception, adjacent to the Atrium. Buffers and solutions stored at ambient temperatures e.g. PBS, TBE x 5, TBS x 10, TBS/Tween x 10, TAE x 50 and Tris/HCL are located on the First floor of the BMRI building, on shelves to the rear of Work Room M101. Bacteriological and Cell Culture media is located in Cold Room MO20, adjacent to the Atrium and opposite to the Media Preparation Lab M021. Antibiotics and Cell Culture products are stored in a Freezer Room, located to the rear of Cold Room M020. Media Preparation Service s olutions for y o ur re s e a rc h ne e ds Media Preparation Service RED UC ING T HE BU RDE N O F P REP ARI NG RO U TINE AN D L ARG E VO L UM ES O F M E D IA CUSTO M IS ED M EDI A Although, a wide selection of media 300+ The School of Life Sciences, University of Warwick The service also supports our Wellesbourne benefits from having its own in-house Media Campus, Crop Centre and Medical School. In an Preparation Service. The primary aim of the average year the team pours in excess of 50,000 service is to reduce the researcher’s burden of agar plates and produces over 12,000 litres of preparing routine and large volumes of media. media. of experience and expertise of creating Making significant time and financial savings, this bacteriological and yeast media, 6,000 litres of custom-made media from scratch. allows research groups and lab personnel to buffers, plus a wide variety of cell culture, This allows modifications to recipes. and dedicate more valuable time to research and antibiotics and associated products. In addition customisation to your exact needs. gathering data. to this over 1,000 bespoke/customised orders This includes 4,500 litres are fulfilled per annum. types are currently produced annually. At times, you may require something a little different. The Media Preparation Team have the Often, this is not an option when commercially commissioned, as it is T UR N A RO UN D TIM ES cost and time prohibitive. The service aims to complete bespoke orders as quickly as possible: solutions usually PRO D UCT RA NG E An extensive range of commonly used buffers, bacteriological and allowing you to focus more time on research within 2-3 days; poured plates usually within 5 days. Sometimes your order may take completion dates are given at the point of ordering. If you have a genuinely urgent request, we will always endeavour to assist. FEED BA CK yeast media, cell culture, antibiotics, amino acid solutions and associated products are routinely prepared and are available as stock items. If products/solutions you or your lab use frequently are not listed in our catalogue, please contact us to We value, welcome and encourage W ATE R STERILE FILTRATION feedback. Constructive comments The preparation of all products (unless otherwise requested) begins with UV treated When autoclaving is not the preferred method of 0.2µm filtered high purity, type 1 pharmaceutical grade 18 MΩ-cm Super-Q water. sterilisation, the Media Preparation Lab has facilities to filter sterilise large volumes of media, using a positive-pressure . A catalogue of stock items prepared can be obtained from the Media Preparation Lab. If you think there are areas where or standards could be improved. Q UALIT Y CO N TRO L filtration system. are always appreciated. our product range, service delivery discuss potential additions to our range. Contact us to discuss your requirements. a little longer due to workload and acquisition of necessary components. Estimated Please let us know. The Media Preparation Service has an The service can filter sterilise up to 20 litres of media at a on-going commitment to producing high- time, in aliquots ranging from 10, 20, 100, 200, 400 and quality and trusted products, with all 800ml routinely. If you require different sized aliquots, this media being fully traceable. This is can easily be accommodated. supported by regular checks and calibration of equipment used within the facility. This includes periodic external PLATE PO U RING validation of autoclaves, balances and Automated plate pouring equipment allows the production pipettors. In addition: CONTACT US We are always happy to discuss individual or lab requirements and any queries you may have concerning our products. of up to 600 standard 90mm agar plates per hour. A range of commonly used plates are always available as stock items. Bespoke and custom prepared plates can be manufactured upon request, at varying thicknesses, if required. Note: when requesting plates, we generally pour in multiples of 30. FILTER STERILISED PRODUCTS Prior to use, multiple samples from each batch are combined with bacteriological broths and incubated to confirm the sample is free from aerobic and anaerobic bacteria, yeast and fungal contamination. After a 10 Working hours are: Mon – Thu 08:00 – 16:00 hrs. Fri 08:00 – 15:30 hrs. day test-period, media is passed and released for use. We also have facilities to pour 60mm petri dishes. AUTOCLAVED PRODUCTS Alternatively, agar can be prepared and kept molten for you Sterility of autoclaved media and buffers is confirmed by the use of to pour different sized plates yourself if this assists. biological indicators during the autoclaving process. Tel: 02476 574240 email: Cerith.Harries@warwick.ac.uk Mark.R.Ward@warwick.ac.uk