Product datasheet Anti-Coronin 1a antibody ab14787 5 Abreviews 1 References 3 Images Overview Product name Anti-Coronin 1a antibody Description Goat polyclonal to Coronin 1a Tested applications WB, ICC/IF Species reactivity Reacts with: Mouse, Rat, Human Predicted to work with: Cow, Dog Immunogen Synthetic peptide: KRLDRLEETVQAK , corresponding to amino acids 449-461 of Human Coronin 1a Run BLAST with Positive control Run BLAST with Lysates prepared from MOLT4 and Jurkat cells. Lysates prepared from transfected HEK293 transiently expressing Coronin 1a. Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. Storage buffer Preservative: 0.02% Sodium Azide Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 Purity Immunogen affinity purified Purification notes Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Clonality Polyclonal Isotype IgG Applications Our Abpromise guarantee covers the use of ab14787 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Abreviews Notes 1 Application Abreviews WB Notes Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). Approx 60kDa band observed in MOLT4 and Jurkat lysates. In transfected HEK293 a band of ~50kDa is observed. ICC/IF Use at an assay dependent concentration. See Abreview. Target Function May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. Tissue specificity Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut. Sequence similarities Belongs to the WD repeat coronin family. Contains 7 WD repeats. Cellular localization Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification. Anti-Coronin 1a antibody images Anti-Coronin 1a antibody (ab14787) at 0.3 µg/ml + Cell lysates prepared from Jurkat cells at 35 µg Predicted band size : 53 kDa Western blot - Coronin 1a antibody (ab14787) 2 ab14787 staining Coronin 1a in human glioblastoma cell line D54MG by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab14787 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC Immunocytochemistry/ Immunofluorescence - conjugated rabbit anti-goat polyclonal, used at Anti-Coronin 1a antibody (ab14787) a 1/50 dilution. Nuclei are counterstained with Image courtesy of an anonymous Abreview. DAPI. ab14787 staining Coronin 1a in rat glioma cell line C6 by Immunocytochemistry/ Immunofluorescence. Cells were fixed in paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 0.5% BSA for 20 minutes at room temperature, then incubated with ab14787 at a 1/50 dilution for 16 hours at 4°C. The secondary used was a TRITC conjugated rabbit anti-goat polyclonal, used at Immunocytochemistry/ Immunofluorescence Anti-Coronin 1a antibody (ab14787) a 1/50 dilution. Nuclei are counterstained with DAPI. Image courtesy of an anonymous Abreview. Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3