Product datasheet
Anti-Mast Cell Chymase antibody ab123510
2 Images
Overview
Product name
Anti-Mast Cell Chymase antibody
Description
Goat polyclonal to Mast Cell Chymase
Tested applications
IHC, ELISA, WB
Species reactivity
Reacts with: Human
Predicted to work with: Cynomolgus Monkey
Immunogen
Synthetic peptide corresponding to Human Mast Cell Chymase aa 96-110 (internal sequence).
Sequence:
C-QFRHPKYNTSTLHHD
Database link: P23946
Run BLAST with
Positive control
Run BLAST with
HEK293 lysate transiently expressing full-length Human Mast Cell Chymase
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity
chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab123510 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
IHC
Use a concentration of 2 - 4 µg/ml.
ELISA
Use at an assay dependent concentration.
Antibody detection limit dilution 1:128,000.
WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 27 kDa.
Target
Function
Major secreted protease of mast cells with suspected roles in vasoactive peptide generation,
extracellular matrix degradation, and regulation of gland secretion.
Tissue specificity
Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria
pigmentosa lesions.
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain.
Cellular localization
Secreted. Cytoplasmic granule. Mast cell granules.
Anti-Mast Cell Chymase antibody images
Immunohistochemical analysis of paraffin
embedded Human Skin tissue
labeling Mast Cell Chymase with ab123510
at 2 ug/ml. Tissue underwent antigen retrieval
in steam with citrate buffer (pH 6.0). The HRPstaining procedure was used for detection.
Immunohistochemistry - Anti-Mast Cell Chymase
antibody (ab123510)
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Lane 1 : Anti-Mast Cell Chymase antibody
(ab123510) at 0.5 µg/ml
Lane 2 : Anti-Mast Cell Chymase antibody
(ab123510) at 1 mg/ml
Lane 3 : Anti-tag antibody at 1/3000 dilution
Lane 1 : HEK293 lysate over expressing
Human CMA1 with a tag at 10 µg
Western blot - Anti-Mast Cell Chymase antibody
(ab123510)
Lane 2 : Mock-transfected HEK293 lysate
Lane 3 : HEK293 lysate over expressing
Human CMA1 with a tag at 10 µg
developed using the ECL technique
Predicted band size : 27 kDa
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