Product datasheet
Anti-Macrophage Scavenger Receptor I antibody
[EPR7536] ab151707
4 Images
Overview
Product name
Anti-Macrophage Scavenger Receptor I antibody [EPR7536]
Description
Rabbit monoclonal [EPR7536] to Macrophage Scavenger Receptor I
Tested applications
WB
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)
corresponding to Human Macrophage Scavenger Receptor I aa 1-100 (N terminal).
Database link: P21757
Positive control
WB: Human placenta, THP1 and HeLa lysates
General notes
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class
antibodies. As a result of this work we are pleased to now offer this antibody in purified
format. We are in the process of updating our datasheets. The purified format is
designated ‘PUR’ on our product labels. If you have any questions regarding this
update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C.
Stable for 12 months at -20°C.
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Purity
Protein A purified
Clonality
Monoclonal
Clone number
EPR7536
Isotype
IgG
1
Applications
Our Abpromise guarantee covers the use of ab151707 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Application notes
Notes
1/1000 - 1/10000. Predicted molecular weight: 50 kDa.
Is unsuitable for ICC,IHC-P or IP.
Target
Function
Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls
during atherogenesis. Two types of receptor subunits exist. These receptors mediate the
endocytosis of a diverse group of macromolecules, including modified low density lipoproteins
(LDL). Isoform III does not internalize actetylated LDL.
Tissue specificity
Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages.
Sequence similarities
Contains 1 collagen-like domain.
Contains 1 SRCR domain.
Cellular localization
Membrane.
Anti-Macrophage Scavenger Receptor I antibody [EPR7536] images
Anti-Macrophage Scavenger Receptor I
antibody [EPR7536] (ab151707) at 1/5000
dilution (purified) + rat placenta at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000
dilution
Predicted band size : 50 kDa
Observed band size : 75 kDa
Blocking buffer: 5% NFDM/TBST
Western blot - Anti-Macrophage Scavenger
Dilution buffer: 5% NFDM/TBST
Receptor I antibody [EPR7536] (ab151707)
2
Anti-Macrophage Scavenger Receptor I
antibody [EPR7536] (ab151707) at 1/1000
dilution (purified) + mouse placenta at 20 µg
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000
dilution
Predicted band size : 50 kDa
Observed band size : 75 kDa
Blocking buffer: 5% NFDM/TBST
Western blot - Anti-Macrophage Scavenger
Dilution buffer: 5% NFDM/TBST
Receptor I antibody [EPR7536] (ab151707)
All lanes : Anti-Macrophage Scavenger
Receptor I antibody [EPR7536] (ab151707)
at 1/1000 dilution (purified)
Lane 1 : THP-1 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
HRP goat anti-rabbit IgG (H+L) at 1/1000
Western blot - Anti-Macrophage Scavenger
dilution
Receptor I antibody [EPR7536] (ab151707)
Predicted band size : 50 kDa
Observed band size : 75 kDa
Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
3
All lanes : Anti-Macrophage Scavenger
Receptor I antibody [EPR7536] (ab151707)
at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : THP1 cell lysate
Lane 3 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Western blot - Anti-Macrophage Scavenger
Predicted band size : 50 kDa
Receptor I antibody [EPR7536] (ab151707)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
4