Product datasheet
Anti-MAdCAM1 antibody ab198277
1 Image
Overview
Product name
Anti-MAdCAM1 antibody
Description
Rabbit polyclonal to MAdCAM1
Tested applications
WB
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthetic peptide corresponding to Human MAdCAM1 (internal sequence). Gene Accssion:
NP_570116
Database link: Q13477
Positive control
Mouse muscle tissue lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab198277 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/200 - 1/1000. Predicted molecular weight: 40 kDa.
Target
Function
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte
traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can
1
bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of
leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin
alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Tissue specificity
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the
small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression
found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart,
placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
Sequence similarities
Contains 2 Ig-like (immunoglobulin-like) domains.
Post-translational
modifications
The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
Cellular localization
Membrane.
Anti-MAdCAM1 antibody images
Anti-MAdCAM1 antibody (ab198277) at
1/300 dilution + Mouse muscle tissue lysate at
40 µg
Predicted band size : 40 kDa
Exposure time : 1 minute
10% SDS-PAGE
Western blot - Anti-MAdCAM1 antibody
(ab198277)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
2