Product datasheet
Anti-M6PR (cation dependent) antibody [EPR7691]
ab134153
2 Images
Overview
Product name
Anti-M6PR (cation dependent) antibody [EPR7691]
Description
Rabbit monoclonal [EPR7691] to M6PR (cation dependent)
Tested applications
WB, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to residues near the C-terminal of Human Mannose 6
Phosphate Receptor (Cation dependent)(P20645).
Positive control
A549 and Human uterus lysates
General notes
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Dissociation constant (KD)
KD = 6.30 x 10 -11 M
Learn more about KD
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EPR7691
Isotype
IgG
1
Applications
Our Abpromise guarantee covers the use of ab134153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 31 kDa.
ICC/IF
1/50 - 1/100.
Application notes
Is unsuitable for Flow Cyt,IHC-P or IP.
Target
Function
Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to
lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to
mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand
complex is transported to an acidic prelyosomal compartment where the low pH mediates the
dissociation of the complex.
Domain
The extracellular domain is homologous to the repeating units (of approximately 147 AA) of the
cation-independent mannose 6-phosphate receptor.
Cellular localization
Lysosome membrane.
Anti-M6PR (cation dependent) antibody [EPR7691] images
All lanes : Anti-M6PR (cation dependent)
antibody [EPR7691] (ab134153) at 1/1000
dilution
Lane 1 : A549 lysates
Lane 2 : Human uterus lysates
Lysates/proteins at 10 µg per lane.
Secondary
HRP labelled goat anti-rabbit at 1/2000
Western blot - Anti-M6PR (cation dependent)
dilution
[EPR7691] antibody (ab134153)
Predicted band size : 31 kDa
2
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other - Anti-M6PR (cation dependent) [EPR7691]
antibody (ab134153)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
3