Product datasheet
Cysteinyl leukotriene ELISA kit ab133042
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Overview
Product name
Cysteinyl leukotriene ELISA kit
Detection method
Colorimetric
Precision
Intra-assay
Sample
n
Mean
SD
CV%
Low
295pg/ml
4.6%
Medium
544pg/ml
4.7%
High
1036pg/ml
4.6%
Sample type
Urine, Tissue Culture Media
Assay type
Competitive
Sensitivity
26.6 pg/ml
Range
78.1 pg/ml - 2500 pg/ml
Recovery
Sample specific recovery
Sample type
Average %
Range
Urine
107.6
%-%
Tissue Culture Media
101
1% - 0.5%
Assay time
3h 00m
Assay duration
Multiple steps standard assay
Species reactivity
Reacts with: Human
Product overview
Abcam’s Cysteinyl Leukotriene in vitro competitive ELISA (Enzyme-Linked Immunosorbent
Assay) kit is designed for the accurate quantitative measurement of Cysteinyl Leukotriene in
Human Urine and Tissue Culture Media.
A goat anti-mouse IgG antibody has been precoated onto 96-well plates. Standards or test
samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-Cysteinyl
Leukotriene antigen and a monoclonal mouse antibody specific to Cysteinyl Leukotriene. After
incubation the excess reagents are washed away. pNpp substrate is added and after a short
incubation the enzyme reaction is stopped and the yellow color generated is read at 405 nm.
The intensity of the yellow coloration is inversely proportional to the amount of Cysteinyl
Leukotriene captured in the plate.
Notes
Leukotrienes are major products of 5-lipoxygenase metabolism of arachidonic acid. Leukotriene
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B4 (LTB4) stimulates leukocyte functions including lysosomal enzyme release, adhesion, and
aggregation of polymorphonuclear leukocytes. LTB4 has been implicated as a potent mediator
of inflammatory diseases and immunoregulation. The cysteinyl leukotrienes (LTC4, LTD4, and
LTE4) are formed by addition of cysteine derivatives to LTA4. The conversion of LTA4 to LTC4
by the enzyme LTC4 synthase limits the rate of cysteinyl leukotriene formation. Cysteinyl
leukotrienes are potent lipid mediators in inflammation, inducing smooth muscle contractions
and increased capillary permeability.
Cross Reactivity
Compound
% Cross Reactivity
LTC4
100
LTD4
115.12
LTE4
62.74
LTB4
1.16
Arachadonic Acid
0.61
PGE2
0.48
6-trans-LTB4
0.45
TXB2
0.28
20-OH-LTB4
<0.2
PGF2α
<0.2
6-keto-PGF1α
<0.2
PGD2
<0.2
15-HETE
<0.2
12-HETE
<0.2
15-HETE
<0.2
Tested applications
Competitive ELISA
Platform
Microplate
Properties
Storage instructions
Please refer to protocols.
Components
1 x 96 tests
20X Wash Buffer Concentrate
1 x 30ml
Assay Buffer
1 x 30ml
Cysteinlyl Leukotriene Standard
1 x 0.5ml
Cysteinyl Leukotriene Alkaline Phosphatase Conjugate
1 x 6ml
Cysteinyl leukotriene Antibody
1 x 6ml
Goat anti-mouse IgG Microplate (12 x 8 wells)
1 unit
Plate Sealer
1 unit
pNpp Substrate
1 x 20ml
Stop Solution
1 x 5ml
Applications
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Our Abpromise guarantee covers the use of ab133042 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Competitive ELISA
Notes
Use at an assay dependent concentration.
Cysteinyl leukotriene ELISA kit images
Representative Standard Curve using
ab133042.
Typical Standard Curve
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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