Product datasheet
Anti-CCR3 antibody ab1667
1 References 2 Images
Overview
Product name
Anti-CCR3 antibody
Description
Goat polyclonal to CCR3
Specificity
Peptide sequence is < 50 % identical to other human chemokine receptors in this region.
Tested applications
ICC, IHC-P, WB
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide: C-VETFGTTSYYDDVGLLCEKADTRALMA, corresponding to N terminal
amino acids 8-34 of Human CCR3.
Run BLAST with
Positive control
Run BLAST with
This anitbody gave a positive signal in human lung, ovary and thymus tissue lysates.
General notes
Human CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively
expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356
amino acids.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
PBS, 1mg/ml BSA, 0.1% sodium azide, pH7.2
Purity
Immunogen affinity purified
Primary antibody notes
Human CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively
expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356
amino acids.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab1667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Notes
ICC
1/150.
IHC-P
1/150.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa
(predicted molecular weight: 53 kDa).
Target
Function
Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and
MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level.
Alternative coreceptor with CD4 for HIV-1 infection.
Tissue specificity
In eosinophils as well as trace amounts in neutrophils and monocytes.
Sequence similarities
Belongs to the G-protein coupled receptor 1 family.
Cellular localization
Cell membrane.
Anti-CCR3 antibody images
Immunocytochemistry on human peripheral
blood cells (acetone fixed cells).
Immunocytochemistry - CCR3 antibody (ab1667)
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All lanes : Anti-CCR3 antibody (ab1667) at 1
µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Ovary (Human) Tissue Lysate - adult
normal tissue (ab30222)
Lane 3 : Thymus (Human) Tissue Lysate adult normal tissue (ab30146)
Lysates/proteins at 10 µg per lane.
Western blot - Anti-CCR3 antibody (ab1667)
Secondary
Rabbit polyclonal to Goat IgG - H&L - PreAdsorbed (HRP) (ab65486) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 53 kDa
Observed band size : 55 kDa
Additional bands at : 100 kDa. We are
unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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