Product datasheet
Anti-Human Ig light chain antibody [7A9] ab1942
1 Abreviews 1 Image
Overview
Product name
Anti-Human Ig light chain antibody [7A9]
Description
Mouse monoclonal [7A9] to Human Ig light chain
Specificity
Human IgA, IgG and IgM.
Tested applications
Conjugation, IHC-P
Species reactivity
Reacts with: Human
Immunogen
Mixture of light chains of human immunoglobulin.
Positive control
IHC-P: FFPE human spleen tissue sections.
General notes
Concentration varies from lot to lot and can be provided on request.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.4
Purity
Protein A purified
Purification notes
Purity tested by electrophoresis.
Clonality
Monoclonal
Clone number
7A9
Myeloma
Sp2/0
Isotype
IgG2a
Applications
Our Abpromise guarantee covers the use of ab1942 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
Conjugation
IHC-P
Application notes
Conjugation: Use at an assay dependent dilution.
1
Specific antibody titer is 1/1000000 by indirect ELISA
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
There are only two types of light chain: kappa and lambda in mammals. Other types of light
chains are found in lower vertebrates as the Ig-Light-Iota chain in Chondrichthyes and Teleostei.
In each antibody, only one type is present and the two chains are identical. Each light chain has
two successive domains: one constant and one variable domain. In humans 60% of light chains
are kappa and 40% lambda,whereas in the mouse 95% of light chains are kappa. The amino
acid sequences of lambda chains vary slightly at a few positions, allowing them to be classified
into subtypes. The number of subtypes varies between species. Monoclonal immunoglobulin free
light chains (FLC) are found in the serum and urine (Bence-Jones protein) of patients with a
number of B-cell proliferative disorders, including multiple myeloma. Changes in serum FLC
concentrations can provide a rapid and sensitive indication of response to treatment.
Cellular localization
Cell Membrane: single-pass type I membrane protein and Secreted
Anti-Human Ig light chain antibody [7A9] images
IHC image of Human Ig light chain staining
in human spleen formalin fixed paraffin
embedded tissue section, performed on a
Leica Bond system using the standard
protocol F. The section was pre-treated using
heat mediated antigen retrieval with sodium
citrate buffer (pH6, epitope retrieval solution
1) for 20 mins. The section was then
incubated with ab1942, 5µg/ml, for 15 mins at
Immunohistochemistry (Formalin/PFA-fixed
room temperature and detected using an
paraffin-embedded sections) - Anti-Human Ig light
HRP conjugated compact polymer system.
chain antibody [7A9] (ab1942)
DAB was used as the chromogen. The
section was then counterstained with
haematoxylin and mounted with DPX.
For other IHC staining systems (automated
and non-automated) customers should
optimize variable parameters such as antigen
retrieval conditions, primary antibody
concentration and antibody incubation times.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
2
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
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