Product datasheet Anti-POP1 antibody ab113272 3 Images Overview Product name Anti-POP1 antibody Description Rabbit polyclonal to POP1 Tested applications IHC-P Species reactivity Reacts with: Human Predicted to work with: Gorilla, Orangutan Immunogen Synthetic peptide, corresponding to a region within amino acids 925-975 of Human POP1 (NP_055844.1). Positive control Breast Carcinoma, Non-Small Cell Lung Cancer, Ovarian Carcinoma, Prostate Carcinoma, Skin Basal Cell, Carcinoma and Testicular Seminoma tissues. Properties Form Liquid Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. Storage buffer Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA Purity Immunogen affinity purified Purification notes ab113272 was affinity purified using an epitope specific to POP1 immobilized on solid support. Clonality Polyclonal Isotype IgG Applications Our Abpromise guarantee covers the use of ab113272 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application IHC-P Abreviews Notes 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with citrate buffer will enhance staining. Target 1 Function Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of RNase MRP. Post-translational modifications Phosphorylated upon DNA damage, probably by ATM or ATR. Cellular localization Nucleus > nucleolus. Anti-POP1 antibody images ab113272 at 1/250 dilution staining POP1 in Formalin-fixed, Paraffin-embedded Human prostate carcinoma tissue by Immunohistochemistry. Detection: DAB staining. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - POP1 antibody (ab113272) ab113272 at 1/250 dilution staining POP1 in Formalin-fixed, Paraffin-embedded Human skin tumor tissue by Immunohistochemistry. Detection: DAB staining. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - POP1 antibody (ab113272) ab113272 at 1/100 dilution staining POP1 in Formalin-fixed Paraffin-embedded Human testicular seminoma tissue by Immunofluorescence. Detection: Redfluorescent Goat anti-Rabbit IgG IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution Immunocytochemistry/ Immunofluorescence - of 1/100. POP1 antibody (ab113272) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery 2 Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 3