Product datasheet
Mouse Isotyping Lateral Flow Kit ab190514
3 Images
Overview
Product name
Mouse Isotyping Lateral Flow Kit
Sample type
Cell culture supernatant, Ascites Fluid, Purified protein
Assay type
Sandwich (qualitative)
Assay time
0h 10m
Assay duration
One step assay
Product overview
Abcam’s one-step immunochromatographic mouse isotyping kit allows for quick and easy
identification of mouse isotypes, subtypes and light chains from a single sample. The sample
used can be purified antibody, cell culture supernatant or ascites fluid. For purified antibody and
cell culture supernatant the test can also be used to determine the relative purity of a hybridoma
cell line. This product is for research use only, not for use in diagnostic applications.
Notes
Lateral-flow membranes are impregnated with separate lines containing antibodies specific for
unique amino acid sequences contained within IgG1, IgG2a, IgG2b, IgG3, IgA, IgM, kappa light
chains and lambda light chains. When the isotyping strips are added to a test tube containing a
diluted mouse immunoglobulin sample, colloidal gold nanoparticles conjugated with antibody
form a soluble immune-complex. The complex is apparent in the form of a red band which allows
users to make a qualitative determination for their specific sample.
Tested applications
Other
Platform
Flow
Properties
Storage instructions
Store at room temperature. Please refer to protocols.
Components
20 tests
Isotyping Mouse Antibody coated test strips (Blue)
1 unit
Isotyping Mouse Antibody coated test strips (Red)
1 unit
Isotyping Mouse Sample Diluent Buffer
1 unit
Cellular localization
Kappa light chain: Cytoplasmic and Secreted Lambda Light chain: Cytoplasmic IgA: Secreted
IgM: Isoform 1: Secreted. During differentiation, B lymphocytes switch from expression of
membrane bound IgM to secretion of IgM. Isoform 2: Cell membrane; Single pass type I
membrane protein. IgG3: Secreted IgG2a: Secreted IgG1: Secreted. Cell membrane; Single1
pass membrane protein IgG2b: Secreted
Applications
Our Abpromise guarantee covers the use of ab190514 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Other
Notes
Use at an assay dependent concentration.
Sandwich ELISA (qualitative)
Mouse Isotyping Lateral Flow Kit images
Blue Strip (kappa, lambda, IgA and IgM)
Lateral Flow assay summary
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Red Strips (G1, IgG2a, IgG2b and IgG3)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Our Abpromise to you: Quality guaranteed and expert technical support
Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
We provide support in Chinese, English, French, German, Japanese and Spanish
Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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