Product datasheet Anti-Dendritic cells antibody [MRC OX-62] (FITC) ab112196 2 References 1 Image Overview Product name Anti-Dendritic cells antibody [MRC OX-62] (FITC) Description Mouse monoclonal [MRC OX-62] to Dendritic cells (FITC) Conjugation FITC. Ex: 493nm, Em: 528nm Tested applications Flow Cyt Species reactivity Reacts with: Rat Immunogen Rat PVG density gradient-enriched veiled cells. Positive control Wistar Rat dendritic cells from thymus. Properties Form Liquid Storage instructions Shipped at 4°C. Store at +4°C. Storage buffer Preservative: 0.02% Sodium azide Constituents: 99% PBS, 0.5% BSA Purity Protein G purified Purification notes ab112196 is purified from ascitic fluid via Protein G Chromatography. Clonality Monoclonal Clone number MRC OX-62 Myeloma NS0 Isotype IgG1 Applications Our Abpromise guarantee covers the use of ab112196 in the following tested applications. The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user. Application Flow Cyt Abreviews Notes Use 2µg for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. 1 Target Relevance Dendritic cells are potent antigen presenting cells that possess the ability to stimulate naive T cells. They are critical for the induction of T cell responses resulting in cell mediated immunity. Within different tissues, dendritic cells differentiate and become active in the taking up and processing of antigens, and their subsequent presentation on the cell surface linked to major histocompatibility (MHC) molecules. Upon appropriate stimulation, dendritic cells undergo further maturation and migrate to secondary lymphoid tissues where they present Ag to T cells and induce an immune response. Anti-Dendritic cells antibody [MRC OX-62] (FITC) images ab112196 at 2µg/106 cells staining Dendritic cells in Rat Thymus cells (Wistar strain) by Flow Cytometry. Percentage of cells stained above control (FITC Mouse IgG1): 82.8%. Flow Cytometry - Dendritic cells antibody [MRC OX-62] (FITC) (ab112196) Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE" Our Abpromise to you: Quality guaranteed and expert technical support Replacement or refund for products not performing as stated on the datasheet Valid for 12 months from date of delivery Response to your inquiry within 24 hours We provide support in Chinese, English, French, German, Japanese and Spanish Extensive multi-media technical resources to help you We investigate all quality concerns to ensure our products perform to the highest standards If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise, please visit http://www.abcam.com/abpromise or contact our technical team. Terms and conditions Guarantee only valid for products bought direct from Abcam or one of our authorized distributors 2