Product datasheet
Anti-Cyclosporin A antibody [CSZ.22] ab8312
1 Abreviews 1 Image
Overview
Product name
Anti-Cyclosporin A antibody [CSZ.22]
Description
Mouse monoclonal [CSZ.22] to Cyclosporin A
Specificity
This antibody recognises Cyclosporin A in buffer solutions and whole blood extracts with Kd =
10-8
Tested applications
Immunoassay, ELISA
General notes
This antibody recognises Cyclosporin A
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS with 0.1% sodium azide, pH 7.4
Purity
Protein G purified
Primary antibody notes
This antibody recognises Cyclosporin A
Clonality
Monoclonal
Clone number
CSZ.22
Isotype
IgG1
Light chain type
unknown
Applications
Our Abpromise guarantee covers the use of ab8312 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Immunoassay
Abreviews
Notes
Use at an assay dependent concentration.
ab8312 recognizes cyclosporin A in buffer solutions and whole blood extracts
with Kd = 108.
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Application
Abreviews
ELISA
Notes
1/1500.
Use at a dilution of 1/1500 for 2 hrs.
Target
Relevance
Cyclosporin is used to prevent rejection following solid organ and bone marrow transplantation .
It has been estimated that cyclosporine is given to more than 90% of children who have received
a kidney transplant in the United States. In addition, the ability of cyclosporin to inhibit T-cell
activation has been shown to have a role in the treatment of diseases such as nephrotic
syndrome, refractory Crohn's disease and ulcerative colitis, biliary cirrhosis, aplastic anemia,
rheumatoid arthritis, myasthenia gravis, and dermatomyositis.
Cellular localization
Cytoplasmic
Anti-Cyclosporin A antibody [CSZ.22] images
ab8312 staining human PBMC, huh-7 cells by
ELISA. Cells were incubated in their
respective growth medium with 10% fetal calf
serum. At the end of the incubation they were
washed with PBS (4°C) and the pellet was
used to determine the CsA content.
1st step: cyclophilin B (ab53870, 1/1500
carbonate pH 8.5) was bound to the plate
ELISA - Cyclosporin A antibody [CSZ.22]
(ab8312)
This image is courtesy of an anonymous Abreview
(overnight 4°C), 2nd step blocking agent, 3rd
step incubation with cell lysate incubated with
CsA or analogs (CsA-OH) overnight,
4th step ab8312 mouse anti CsA, 1/500 for 2
hours at 37°C
5th step ab6729 (1/2000) Alkalline
phosphatase reading at 405 nm
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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