Product datasheet
Anti-Orai1 antibody ab86748
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Overview
Product name
Anti-Orai1 antibody
Description
Rabbit polyclonal to Orai1
Tested applications
IHC-P, WB
Species reactivity
Reacts with: Mouse, Human
Immunogen
Synthetic peptide from the center region of Human Orai1 (NP_116179.2), conjugated to KLH.
Positive control
A375 cell lysate, Mouse testis tissue lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Purity
Immunogen affinity purified
Purification notes
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab86748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
1/50 - 1/100.
WB
1/1000. Predicted molecular weight: 33 kDa.
Target
Function
Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx
following depletion of intracellular Ca(2+) stores and channel activation by the Ca(2+) sensor,
STIM1. CRAC channels are the main pathway for Ca(2+) influx in T-cells and promote the
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immune response to pathogens by activating the transcription factor NFAT.
Involvement in disease
Defects in ORAI1 are the cause of immune dysfunction with T-cell inactivation due to calcium
entry defect type 1 (IDTICED1) [MIM:612782]. IDTICED1 is an immune disorder characterized
by recurrent infections, impaired T-cell activation and proliferative response, decreased T-cell
production of cytokines, and normal lymphocytes counts and serum immunoglobulin levels. In
surviving patients ectodermal dysplasia with anhydrosis and non-progressive myopathy may be
observed.
Sequence similarities
Belongs to the Orai family.
Cellular localization
Cell membrane.
Anti-Orai1 antibody images
All lanes : Anti-Orai1 antibody (ab86748) at
1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : Mouse testis tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size : 33 kDa
Western blot - Anti-Orai1 antibody (ab86748)
Immunohistochemical staining of formalinfixed and paraffin-embedded human prostate
carcinoma with ab86748 followed by a HRPconjugated secondary antibody and DAB
staining.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-Orai1 antibody
(ab86748)
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Terms and conditions
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