Product datasheet
Anti-GATA3 antibody ab89776
4 Images
Overview
Product name
Anti-GATA3 antibody
Description
Mouse monoclonal to GATA3
Tested applications
WB, ELISA
Species reactivity
Reacts with: Human
Immunogen
Recombinant fragment: LGSHHTASPW NLSPFSKTSI HHGSPGPLSV YPPASSSSLS
GGHASPHLFT FPPTPPKDVS PDPSLSTPGS AGSARQDEKE CLKYQVPLPD SMKLESSH,
corresponding to amino acids 103-200 of human GATA3 (NP_001002295), with a proprietary
tag
Run BLAST with
Positive control
Run BLAST with
The immunogen; HeLa nuclear extract; Jurkat cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw
cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Applications
Our Abpromise guarantee covers the use of ab89776 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 48 kDa.
ELISA
Use at an assay dependent dilution.
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Target
Function
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta
genes. Binds to the consensus sequence 5'-AGATAG-3'.
Tissue specificity
T-cells and endothelial cells.
Involvement in disease
Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal
dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
Sequence similarities
Contains 2 GATA-type zinc fingers.
Cellular localization
Nucleus.
Form
There are 2 isoforms produced by alternative splicing.
Anti-GATA3 antibody images
Anti-GATA3 antibody (ab89776) at 1 µg/ml +
immunogen at 0.2 µg
Predicted band size : 48 kDa
Western blot - GATA3 antibody (ab89776)
Anti-GATA3 antibody (ab89776) at 1 µg/ml +
HeLa nuclear extract at 50 µg
Predicted band size : 48 kDa
Observed band size : 55 kDa
Western blot - GATA3 antibody (ab89776)
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Anti-GATA3 antibody (ab89776) at 1 µg/ml +
Jurkat cell lysate at 50 µg
Predicted band size : 48 kDa
Observed band size : 48 kDa
Additional bands at : 37 kDa. We are
unsure as to the identity of these extra bands.
Western blot - GATA3 antibody (ab89776)
Detection limit for ab89776 is approximately
0.03 ng/ml as a capture antibody.
ELISA - GATA3 antibody (ab89776)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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