Product datasheet
Anti-ZAP70 (phospho Y318) antibody ab194792
2 Images
Overview
Product name
Anti-ZAP70 (phospho Y318) antibody
Description
Rabbit polyclonal to ZAP70 (phospho Y318)
Tested applications
IHC-P, WB, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide corresponding to Human ZAP70 (phospho Y318).
Database link: P43404
Positive control
Jurkat cell extracts. HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long
term. Avoid freeze / thaw cycle.
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab194792 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-P
1/50 - 1/200.
WB
1/500 - 1/2000. Predicted molecular weight: 70 kDa.
ICC/IF
1/50 - 1/100.
Target
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Cellular localization
Cytoplasm. Cell membrane; Peripheral membrane protein. Note=In quiescent T-lymphocytes, it
is cytoplasmic. Upon TCR activation, it is recruited at the plasma membrane by interacting with
CD247/CD3Z. Co-localizes together with RHOH in the immunological synapse. RHOH is
required for its proper localization to the cell membrane and cytoskeleton fractions in the
thymocytes (By similarity)
Anti-ZAP70 (phospho Y318) antibody images
All lanes : Anti-ZAP70 (phospho Y318)
antibody (ab194792) at 1/500 dilution
Lane 1 : Jurkat cell extract with peptide
Lane 2 : Jurkat cell extract
Predicted band size : 70 kDa
Western blot - Anti-ZAP70 (phospho Y319)
antibody (ab194792)
Immunofluorescent staining of methanol fixed
HeLa cells, labeling ZAP70 with ab194792 at
1/50 dilution.
Immunocytochemistry/ Immunofluorescence Anti-ZAP70 (phospho Y319) antibody (ab194792)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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