Product datasheet
Anti-CD3 antibody ab5690
36 Abreviews 63 References 6 Images
Overview
Product name
Anti-CD3 antibody
Description
Rabbit polyclonal to CD3
Tested applications
IHC-Fr, WB, IHC-P, IHC-FoFr, ICC/IF
Species reactivity
Reacts with: Mouse, Rat, Human
Immunogen
Synthetic peptide:
KAKAKPVTRGAGA
, corresponding to amino acids 156-168 of Human CD3 Epsilon chain.
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Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: PBS, pH 7.4
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab5690 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
Notes
IHC-Fr
1/100. PubMed: 18628996
WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 23 kDa.
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC
staining protocol. The recommended starting incubation time is 10min.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 20126467
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Application
Abreviews
ICC/IF
Notes
Use at an assay dependent concentration. PubMed: 23874197
Target
Function
The CD3 complex mediates signal transduction.
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive Tcell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of
severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of
rare congenital disorders characterized by impairment of both humoral and cell-mediated
immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent,
persistent infections by opportunistic organisms. The common characteristic of all types of SCID
is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
Sequence similarities
Contains 1 ITAM domain.
Cellular localization
Membrane.
Anti-CD3 antibody images
ab5690 at 1/100 staining mouse lymph node
tissue sections by IHC-Fr. The tissue was
acetone fixed and blocked with serum before
incubation with the antibody for 1 hour. An
Alexa Fluor ® 488 conjugated goat anti-rabbit
antibody was used as the secondary.
Immunohistochemistry (Frozen sections) - CD3
antibody (ab5690)
This image is courtesy of an Abreview submitted by
Dr Alexandre Garin
Anti-CD3 antibody (ab5690) + Jurkat Cell
Lysate
Predicted band size : 23 kDa
Observed band size : 22 kDa
Western blot - Anti-CD3 antibody (ab5690)
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No positive immunostaining for CD3ε, a pan
T cell marker (ab5690), was detected in the
corneas of scrambled siRNA-treated mice (A)
at 5 days p.i. In contrast, positive
immunostaining (red) was observed in the
peripheral cornea of HIF-1α silenced animals
(B). The control sections shown in (C) and (D)
were immunostained with species-specific
IgG and were positive for SYTOX Green
Immunocytochemistry/ Immunofluorescence -
nuclear stain only. Images shown are
Anti-CD3 antibody (ab5690)
representative of three independent
experiments each with three mice per group.
Magnification = 180×; inset = 335×.
ab5690 staining rat spleen tissue sections by
IHC-P. Sections were formaldehyde fixed
and subjected to heat mediated antigen
retrieval in sodium citrate buffer (10mM
Sodium Citrate, 0.05% Tween 20, pH 6.0)
prior to blocking in 5% normal goat serum +
1% BSA for 2 hours at 37°C. The primary
antibody was diluted 1/200 and incubated
with the sample for 16 hours at 4°C. A
Immunohistochemistry (Formalin/PFA-fixed
biotinylated goat anti-rabbit antibody, diluted
paraffin-embedded sections) - CD3 antibody
1/100, was used as the secondary.
(ab5690)
This image is courtesy of an Abreview submitted by
Dr Reza Jalili
ab5690 staining CD3 in the lymphatic nodule
of rat spleen by Immunohistochemistry
(Formalin/PFA-fixed paraffin-embedded
sections). Tissue was fixed with formaldehyde
and a heat mediated antigen retrieval step
was performed using citrate buffer pH 6.0.
Samples were then blocked with 1% BSA for
25 minutes at 20°C followed by incubation
with the primary antibody at a 1/100 dilution
Immunohistochemistry (Formalin/PFA-fixed
for 1 hour at 20°C. A biotin-conjugated goat
paraffin-embedded sections) - CD3 antibody
anti-rat polyclonal was used undiluted as the
(ab5690)
Image courtesy of an anonymous Abreview.
secondary antibody.
CD3 positive cells are mainly distributed in
PALS (T-cell region), while sporadic CD3
positive cells are identified in lymphoid follicle
and germinal center (B-cell region).
3
ab5690 staining CD3 in human lymph node
tissue by Immunohistochemistry
(Formalin/PFA-fixed paraffin-embedded
tissue sections). The sections were fixed in
paraformaldehyde and subjected to heatmediated antigen retrieval in citric buffer pH
6.0, prior to blocking with 10% serum for 1
hour at 20°C. The primary antibody was
diluted 1/100 and incubated with the sample
Immunohistochemistry (Formalin/PFA-fixed
for 12 hours at 4°C. An HRP-conjugated goat
paraffin-embedded sections) - CD3 antibody
anti-rabbit polyclonal was used as the
(ab5690)
secondary antibody, diluted 1/200.
This image is courtesy of an Anonymous Abreview.
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