Product datasheet
Anti-MHC Class I H2 Kd + H2 Dd antibody [34-7-23S]
ab131404
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Overview
Product name
Anti-MHC Class I H2 Kd + H2 Dd antibody [34-7-23S]
Description
Mouse monoclonal [34-7-23S] to MHC Class I H2 Kd + H2 Dd
Specificity
ab131404 is specific for cells expressing the H-2K and/or H-2D antigen coded for by the d
haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant
haplotypes demonstrates that it detects a previously unidentified serological specificity similar to
H-2.27, H-2.28, H-2.29. ab131404 can be used to quantitate or eliminate cells bearing the H2Kd and/or H-2Dd antigen from the appropriate strains of mice. This antibody will also react with
H-2Kb and H-2q.
Tested applications
Functional Studies
Species reactivity
Reacts with: Mouse
Immunogen
Mouse B6 x DBA/2 prepared from C3H/He spleen cells
Positive control
BALB/c thymus, lymph node and bone marrow cell suspension
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: 99% PBS
Purity
Purified IgM
Clonality
Monoclonal
Clone number
34-7-23S
Myeloma
Sp2/0-Ag14
Isotype
IgM
Applications
Our Abpromise guarantee covers the use of ab131404 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
Abreviews
Functional Studies
Notes
1/20. Used in cytotoxicity analysis.
Target
Relevance
The "classical" MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene
complex and consist of heterodimers of highly polymorphic alpha chains noncovalently
associated with the invariant beta 2-microglobulin. These antigens are expressed on most
nucleated cells but expression varies on different cell types. MHC Class I molecules present
endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.
MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative
selection of CD8+ T cells during T cell ontogeny.
Cellular localization
Type I membrane protein
Anti-MHC Class I H2 Kd + H2 Dd antibody [34-7-23S] images
Cytoxicity analysis of ab131404 at 1/20
dilution using Mouse (BALB/c) thymus, lymph
node and bone marrow cells.
Functional Studies - Anti-MHC Class I H2 Kd +
H2 Dd antibody [34-7-23S] (ab131404)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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