Product datasheet
Anti-LAX1 antibody [EP8753] ab133759
7 Images
Overview
Product name
Anti-LAX1 antibody [EP8753]
Description
Rabbit monoclonal [EP8753] to LAX1
Tested applications
WB, IHC-P, Flow Cyt
Species reactivity
Reacts with: Human
Immunogen
Synthetic peptide corresponding to a region within the extracellular domain of Human LAX1.
(Q8IWV1)
Positive control
Raji cell lysate, Jurkat cell lysate, THP1 cell lysate, Daudi cell lysate, HuT78 cell lysate, HeLa cell
lysate, Human spleen tissue.
General notes
Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the
following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with
these species. Please contact us for more information.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)
KD = 2.60 x 10 -11 M
Learn more about KD
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 0.05% BSA
Purity
Tissue culture supernatant
Clonality
Monoclonal
Clone number
EP8753
Isotype
IgG
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Applications
Our Abpromise guarantee covers the use of ab133759 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
Abreviews
WB
Notes
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular
weight: 44 kDa).
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6
before commencing with IHC staining protocol.
Flow Cyt
1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype
control with this antibody.
Application notes
Is unsuitable for ICC or IP.
Target
Relevance
LAX1 negatively regulates TCR (T-cell antigen receptor)-mediated signaling in T-cells and BCR
(B-cell antigen receptor)-mediated signaling in B-cells.
Cellular localization
Cell Membrane; Single-pass type III membrane protein.
Anti-LAX1 antibody [EP8753] images
Immunohistochemical analysis of paraffin
embedded Human spleen tissue labelling
LAX1 with ab133759 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections) - Anti-LAX1 antibody
[EP8753] (ab133759)
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All lanes : Anti-LAX1 antibody [EP8753]
(ab133759) at 1/1000 dilution
Lane 1 : Raji cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : THP1 cell lysate
Lane 4 : Daudi cell lysate
Lane 5 : HuT 78 cell lysate
Lane 6 : HeLa cell lysate
Western blot - Anti-LAX1 antibody [EP8753]
Lysates/proteins at 10 µg per lane.
(ab133759)
Secondary
Goat anti-rabbit HRP conjugated antibody at
1/2000 dilution
Predicted band size : 44 kDa
Immunohistochemical analysis using
ab133759 showing positive staining in
Normal colon tissue.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections)-Anti-LAX1 antibody
[EP8753](ab133759)
Immunohistochemical analysis using
ab133759 showing positive staining in
Normal tonsil tissue.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections)-Anti-LAX1 antibody
[EP8753](ab133759)
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Immunohistochemical analysis using
ab133759 showing positive staining in
Normal kidney tissue.
Immunohistochemistry (Formalin/PFA-fixed
paraffin-embedded sections)-Anti-LAX1 antibody
[EP8753](ab133759)
ab133759 staining LAX1 (red) in live Jurkat
cells (1x106 cells/sample) by Flow Cytometry.
Samples were incubated with primary
antibody for 60 minutes at room temperature.
Cells were then incubated with a fluorescentconjugated secondary antibody for 30
minutes in darkness.
Green: Rabbit IgG (Negative)
Flow Cytometry - Anti-LAX1 antibody [EP8753]
(ab133759)
Blocking buffer: 3% FBS in 1X PBS
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
Other-Anti-LAX1 antibody [EP8753](ab133759)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Replacement or refund for products not performing as stated on the datasheet
Valid for 12 months from date of delivery
Response to your inquiry within 24 hours
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Extensive multi-media technical resources to help you
We investigate all quality concerns to ensure our products perform to the highest standards
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If the product does not perform as described on this datasheet, we will offer a refund or replacement. For full details of the Abpromise,
please visit http://www.abcam.com/abpromise or contact our technical team.
Terms and conditions
Guarantee only valid for products bought direct from Abcam or one of our authorized distributors
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