Anti-RNF20 antibody ab70495 Product datasheet 1 Abreviews 3 Images

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Product datasheet
Anti-RNF20 antibody ab70495
1 Abreviews 1 References 3 Images
Overview
Product name
Anti-RNF20 antibody
Description
Rabbit polyclonal to RNF20
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has
not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
WB, IP, ICC/IF
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey,
Gorilla, Chinese Hamster, Orangutan
Immunogen
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human RNF20
Positive control
Whole cell lysate from HeLa and 293T cells
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Our Abpromise guarantee covers the use of ab70495 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application
WB
Abreviews
Notes
1/2000 - 1/10000. Detects a band of approximately 140 kDa (predicted
molecular weight: 114 kDa).
IP
Use at 2 µg/mg of lysate.
ICC/IF
1/1000.
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Target
Function
Component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates
monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific
tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and
'Lys-79' methylation (H3K4me and H3K79me, respectively). It thereby plays a central role inb
histone code and gene regulation. The RNF20/40 complex forms a H2B ubiquitin ligase
complex in cooperation with the E2 enzyme UBE2A or UBE2B; reports about the cooperation
with UBE2E1/UBCH are contradictory. Required for transcriptional activation of Hox genes.
Recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as
a transcriptional coactivator. Mediates the polyubiquitination of isoform 2 of PA2G4 in cancer
cells leading to its proteasome-mediated degradation.
Tissue specificity
Expressed in the normal brain and also in malignant gliomas (at protein level).
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the BRE1 family.
Contains 1 RING-type zinc finger.
Cellular localization
Nucleus.
Anti-RNF20 antibody images
All lanes : Anti-RNF20 antibody (ab70495)
at 0.1 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at
50 µg
Lane 2 : Whole cell lysate from HeLa cells at
Western blot - RNF20 antibody (ab70495)
15 µg
Lane 3 : Whole cell lysate from HeLa cells at
5 µg
Lane 4 : Whole cell lysate from 293T cells at
50 µg
developed using the ECL technique
Predicted band size : 114 kDa
Observed band size : 140 kDa
Additional bands at : 171 kDa,280 kDa.
We are unsure as to the identity of these extra
bands.
Exposure time : 30 seconds
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Detection of Human RNF20 by
Immunoprecipitation in Whole cell lysate from
HeLa cells (1 mg for IP, 20% of IP loaded)
using ab70495 at 3 µg/mg lysate.
Subsequent RNF20 WB detection was
performed using ab70495 at 1 µg/ml.
Immunoprecipitation - RNF20 antibody (ab70495)
ab70495 (1/1000) detecting RNF20 in HeLa
cells (green). Cells were fixed in
paraformaldehyde, permeabilised with 0.5%
Triton X100/PBS and counterstained with
DAPI in order to highlight the nucleus (red).
Please refer to Abreview for further
Immunocytochemistry/ Immunofluorescence -
experimental details.
RNF20 antibody (ab70495)
This image is courtesy of an Abreview submitted by
Dr Kirk McManus
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